Strain Data Sheet

RBRC11539

Strain Information

Image
BRC No.RBRC11539
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Awat2<em1Akih>
Former Common nameAwat2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Akio Khara in 2021. Mice were generated using C57BL/6J with the CRISPR/Cas9. C57BL/6J genetic background.
Strain descriptionAwat2 mutant mice. 12 bp insertion at exon 5 of the Awat2 gene.
Colony maintenance
References
Erratum: Diverse meibum lipids produced by Awat1 and Awat2 are important for stabilizing tear film and protecting the ocular surface.
Sawai M, Watanabe K, Tanaka K, Kinoshita W, Otsuka K, Miyamoto M, Sassa T, Kihara A
iScience, 24, 103160 (2021). 34622178

Diverse meibum lipids produced by Awat1 and Awat2 are important for stabilizing tear film and protecting the ocular surface.
Sawai M, Watanabe K, Tanaka K, Kinoshita W, Otsuka K, Miyamoto M, Sassa T, Kihara A
iScience, 24, 102478 (2021). 34113821

Health Report

Examination Date / Room / Rack2024/08/19Room:4-ARack:JSentinel mouse program
2024/05/20Room:4-ARack:JSentinel mouse program
2024/02/19Room:4-ARack:JSentinel mouse program
2023/11/20Room:4-ARack:JSentinel mouse program
2023/08/21Room:4-ARack:JSentinel mouse program
2023/05/22Room:4-ARack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Awat2
MGI:3045345		acyl-CoA wax alcohol acyltransferase 2XAwat2<em1Akih> endonuclease-mediated mutation 1, Akio Kihara

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAS. pyogenes Cas9* * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan).
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkio Kihara (Hokkaido University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data