Strain Data Sheet

RBRC11547

Strain Information

Image
BRC No.RBRC11547
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Ntrk1<tm1(E495K)Tkato>
Former Common nameB6J-Ntrk1 (E495K) cKI neo/+
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tadafumi Kato, RIKEN CBS in 2021. This strain was generated using CMTI-2 ES cells derived from C57BL/6J. C57BL/6 genetic background.
Strain descriptionA damaging missense mutation E492K (rs144901788) reported in humans was introduced in the mouse Ntrk1 (TrkA) genomic region. A PGK-neo cassette was not removed in this strain. To express the mutated E492K, this strain should be crossed to Flp and Cre strains.
Colony maintenance
ReferencesTransl. Psychiatry, 10, 407 (2020). 33235206

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ntrk1neurotrophic tyrosine kinase, receptor, type 13Ntrk1<tm1(E495K)Tkato>endonuclease-mediated mutation 1, Tadafumi Kato

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox2272phage P1 loxP variant3lox2272

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA3

Ordering Information

Donor DNABacteriophage P1 loxP配列 lox2272, 酵母 Frt, マウス PGKプロモーター, 大腸菌Neomycin 耐性遺伝子, シミアンウイルス４０ SV40 polyA配列
Research applicationCre/loxP system
FLP/frt system
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Transl. Psychiatry, 10, 407 (2020).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorTadafumi Kato (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data