Strain Data Sheet

RBRC11551

Strain Information

Image
BRC No.RBRC11551
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Ntrk1<em4Tkato>/KN9
Former Common nameB6J-Ntrk1 (CRISPR, KO); 13bΔ/+
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tadafumi Kato, RIKEN CBS in 2021. This strain was generated using the CRISPR/Cas9. C57BL/6J genetic background.
Strain description13bp deletion at exon 12 of the Ntrk1 gene, which results in frame-shift with premature termination.
Colony maintenance
ReferencesTransl. Psychiatry, 10, 407 (2020). 33235206
Sci. Rep., 6:34703 (2016). 27698470

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ntrk1neurotrophic tyrosine kinase, receptor, type 13Ntrk1<em4Tkato>endonuclease-mediated mutation 4, Tadafumi Kato

Ordering Information

Donor DNAStreptococcus pyogenes Cas9 mRNA, Streptococcus pyogenes sgRNA, マウス single strand oligo DNA (a part of Ntrk1 gene)
Research applicationMouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Transl. Psychiatry, 10, 407 (2020).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorTadafumi Kato (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data