Strain Data Sheet
RBRC11555
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11555 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Hcrt<tm1.1(FLP)Ksak> |
| Former Common name | Orexin-Flippase |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Depsoted by Akihiro Yamanaka, Nagoya University in 2021. This strain was generated RENKA ES cells derived from C57BL/6N. |
| Strain description | EGFP/2A/FLP knockin mice at the translation initiation codon of the Hcrt gene. |
| Colony maintenance | Heterozygote x Wild-type [or crossing to C57BL/6JJcl] |
| References | Elife, 8:e44927 (2019). 31159922 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/02/26Room:4-CRack:F 2023/11/27Room:4-CRack:F 2023/08/28Room:4-CRack:F 2023/05/29Room:4-CRack:F 2023/02/27Room:4-CRack:F 2022/11/29Room:4-CRack:F 2022/08/31Room:4-CRack:F 2022/08/29Room:4-CRack:F |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)11EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLPeyeast flippase recombinase11FLPe Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHgrowth hormone polyA (human)11GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter T2AThosea asigna virus 2A peptide (insect)11T2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)11nls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site11rox |
Ordering Information | |
|---|---|
| Donor DNA | Aequorea victoria EGFP cDNA, Thosea asigna virus 2A peptide (insect), SV40 nls, Saccharomyces cerevisiae Flpe recombinase, Bacteriophage D6 rox site, human GH polyA signal, mouse Hcrt genomic DNA |
| Research application | FLP/frt system Fluorescent Proteins/lacZ System Neurobiology Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, Recipient shall inform the DEPOSITOR in advance, and shall discuss and agree on the handing thereof. The RECIPIENT shall contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of BIOLOGICAL RESOURCE, and shall obtain a prior written consent from the DEPOSITOR. |
| Depositor | Akihiro Yamanaka (Nagoya University) |
| Strain Status |  Live mice Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |