Strain Data Sheet
RBRC11557
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11557 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129S-Ostn<tm1Ncvc> |
| Former Common name | Osteocrin(Ostn)LacZ/LacZ or Ostn knockout mouse, B6.129S-Ostn<tm1a(KOMP)Nmoc> |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | |
| Strain development | Deposited and developed by Naoki Mochizuki and Haruko Takano, National Cerebral and Cardiovascular Center Research Institute. This strain was generated using R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> and backcrossed to C57BL/6J for 9 generations. C57BL/6J congenic line. |
| Strain description | LacZ knockin mice at the Ostn locus. |
| Colony maintenance | |
| References | Mechanical load regulates bone growth via periosteal Osteocrin. Watanabe-Takano H, Ochi H, Chiba A, Matsuo A, Kanai Y, Fukuhara S, Ito N, Sako K, Miyazaki T, Tainaka K, Harada I, Sato S, Sawada Y, Minamino N, Takeda S, Ueda H R, Yasoda A, Mochizuki N Cell Rep., 36(2):109380 (2021). 34260913 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/06/10Room:3-6Rack:ASentinel mouse program 2024/03/11Room:3-6Rack:ASentinel mouse program 2023/12/11Room:3-6Rack:ASentinel mouse program 2023/09/11Room:3-6Rack:ASentinel mouse program 2023/07/18Room:3-6Rack:AAdditional tests 2023/06/13Room:3-6Rack:ASentinel mouse program 2023/03/13Room:3-6Rack:ASentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| En2 | En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence) | 16 | En2 | ||||
| Frt | yeast FRT (flippase recombination target) site | 16 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 16 | Frt | ||||
| Ires | internal ribosomal entry site (EMCV) | 16 | |||||
| Ostn MGI:2677164 | osteocrin | 16 | Ostn<tm1Ncvc> MGI:6877071 | targeted mutation 1, National Cerebral and Cardiovascular Center | |||
| lacZ | beta-galactosidase (E. coli) | 16 | |||||
| loxP | phage P1 loxP | 16 | loxP | ||||
| loxP | phage P1 loxP | 16 | loxP | ||||
| loxP | phage P1 loxP | 16 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 16 | |||||
| SV40 polyA | 16 | ||||||
| SV40 polyA | 16 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | yeast FRT (flippase recombination target) sites, attB1, Mouse En2 intron (Mouse En2 intron Splicing acceptor), Ires (internal ribosomal entry site (EMCV)), E. coli beta-galactosidase, Simian Virus 40 PolyA signal, Bacteriophage P1 LoxP sites, E. coli Neomycin resistant gene, attB2, mouse Ostn genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and KOMP Repository as the source of the original allele and mice are requested. |
| Depositor | Naoki MOCHIZUKI (National Cerebral and Cardiovascular Center) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |