Strain Information | |
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Image | |
BRC No. | RBRC11575 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | C57BL/6N-Gt(ROSA)26Sor<tm1(CAG-G-CaMP9a,-R-CaMP2)Hbto> |
Former Common name | G-CaMP9a/R-CaMP2 KI mice |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Deposited by Haruhiko Bito and Masayuki Sakamoto, The Universtiy of Tokyo in 2022. This strain was generated using RENKA (ES cell) derived from C57BL/6NCrlCrlj. C57BL/6N genetic background. |
Strain description | G-CaMP9a and R-CaMP2 reporter mice under the control of Flp/FRT and Cre/loxP recombination, respectively.R-CaMP2 expression can be regulated by tTA/rtTA, but its expression is relatively weak. |
Colony maintenance | |
References | In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. Masayuki Sakamoto, Keisuke Ota, Yayoi Kondo, Michiko Okamura, Hajime Fujii, Haruhiko Bito STAR Protoc., 3(2):101421 (2022). 35693213A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Masayuki Sakamoto, Masatoshi Inoue, Atsuya Takeuchi, Shigetaka Kobari, Tatsushi Yokoyama, Shin-Ichiro Horigane, Sayaka Takemoto-Kimura, Manabu Abe, Kenji Sakimura, Masanobu Kano, Kazuo Kitamura, Hajime Fujii, Haruhiko Bito Cell Rep., Methods 2, 100168 (2022). 35474964 |
Health Report | |
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Examination Date / Room / Rack | 2024/05/27Room:4-CRack:BSentinel mouse program 2024/02/26Room:4-CRack:BSentinel mouse program 2023/11/27Room:4-CRack:BSentinel mouse program 2023/08/28Room:4-CRack:BSentinel mouse program 2023/05/29Room:4-CRack:BSentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
GCaMP9a | RSET tag, ckkap, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA | 6 | GCaMP9a | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
GH | Growth hormone polyA (human) | 6 | GH | ||||
Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1(CAG-G-CaMP9a,-R-CaMP2)Hbto> | targeted mutation 1, Haruhiko Bito | |||
His3 | His3 (S. cerevisiae) | 6 | His3 | ||||
RCaMP2 | His-tag, T7-tag, Xpress-tag, ckkap, cpApple(circularly permuted red fluorescent protein (mApple)) cDNA, rat calmodulin (CaM) cDNA | 6 | RCaMP2 | ||||
TRE3G | Tet-On 3G (transactivator gene) | 6 | TRE3G | ||||
WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | 6 | WPRE | ||||
WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | 6 | WPRE | ||||
loxP | phage P1 loxP | 6 | loxP | ||||
loxP | phage P1 loxP | 6 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 6 | mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter | ||||
rox | Bacteriophage D6 rox site | 6 | rox | ||||
rox | Bacteriophage D6 rox site | 6 | rox | ||||
SV40 polyA | 6 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | attB1, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites,yeast FRT (flippase recombination target) sites, 2 x pA, Stop (SV40 polyA), G-CaMP9a(RSET tag, ckkap, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE), rabbit beta globin polyA signal, E.coli TRE3G(Tet-On 3G, transactivator gene), R-CaMP2 (His-tag, T7-tag, Xpress-tag, Ca2+/CaM-binding peptide derived from rat CaMKKα Val438–Phe463 (ckkap), Discosoma sp. cpApple(circularly permuted red fluorescent protein (mApple)) cDNA, rat calmodulin (CaM) cDNA), human GH polyA, Phage D6 rox sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli neomycin resistance gene, synthetic polyA, attB2, yeast His3, mouse Gt(ROSA)26Sor genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Haruhiko Bito (The University of Tokyo) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month May 2023 |
BRC mice in Publications |
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