Strain Data Sheet

RBRC11575

Strain Information

Image
BRC No.RBRC11575
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Gt(ROSA)26Sor<tm1(CAG-G-CaMP9a,-R-CaMP2)Hbto>
Former Common nameG-CaMP9a/R-CaMP2 KI mice
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Haruhiko Bito and Masayuki Sakamoto, The Universtiy of Tokyo in 2022. This strain was generated using RENKA (ES cell) derived from C57BL/6NCrlCrlj. C57BL/6N genetic background.
Strain descriptionG-CaMP9a and R-CaMP2 reporter mice under the control of Flp/FRT and Cre/loxP recombination, respectively.R-CaMP2 expression can be regulated by tTA/rtTA, but its expression is relatively weak.
Colony maintenance
ReferencesCell Rep., Methods 2, 100168 (2022). 35474964
STAR Protoc., 3(2):101421 (2022). 35693213

Health Report

Examination Date / Room / Rack2024/02/26Room:4-CRack:B
2023/11/27Room:4-CRack:B
2023/08/28Room:4-CRack:B
2023/05/29Room:4-CRack:B
2023/02/27Room:4-CRack:B
2022/11/29Room:4-CRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-G-CaMP9a,-R-CaMP2)Hbto>targeted mutation 1, Haruhiko Bito

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP9aRSET tag, ckkap, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA6GCaMP9aCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
His3His3 (S. cerevisiae)6His3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RCaMP2His-tag, T7-tag, Xpress-tag, ckkap, cpApple(circularly permuted red fluorescent protein (mApple)) cDNA, rat calmodulin (CaM) cDNA6RCaMP2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TRE3GTet-On 3G (transactivator gene)6TRE3G

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site6rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site6rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Ordering Information

Donor DNAattB1, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites,yeast FRT (flippase recombination target) sites, 2 x pA, Stop (SV40 polyA), G-CaMP9a(RSET tag, ckkap, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE), rabbit beta globin polyA signal, E.coli TRE3G(Tet-On 3G, transactivator gene), R-CaMP2 (His-tag, T7-tag, Xpress-tag, Ca2+/CaM-binding peptide derived from rat CaMKKα Val438–Phe463 (ckkap), Discosoma sp. cpApple(circularly permuted red fluorescent protein (mApple)) cDNA, rat calmodulin (CaM) cDNA), human GH polyA, Phage D6 rox sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli neomycin resistance gene, synthetic polyA, attB2, yeast His3, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorHaruhiko Bito (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data