Strain Information | |
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Image | |
BRC No. | RBRC11578 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | C.Cg-Hras<tm1.1(G12S)Tumg> |
Former Common name | |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Deposited by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2021. BRUCE-4 ES cells were used. Mice were backcrossed to BALB/c for more than 10 generations. |
Strain description | Knock-in mutants in which a point mutation (G12S) was introduced at Exon 1 of the Hras gene. Floxed cDNA/stop cassette and Frt-flnaked Neo cassette were removed by crossing with Cre mice. BALB/c congenic strain. C57BL/6 congenic strain is also available as RBRC10866. |
Colony maintenance | |
References | EBioMedicine, 27:138-150 (2018). 29254681 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/26Room:4-BRack:E |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP site, mouse Hras genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EBioMedicine. 27:138-150.(2018) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use base on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Yoko Aoki (Tohoku University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |