Strain Data Sheet

RBRC11579

Strain Information

Image
BRC No.RBRC11579
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Gt(ROSA)26Sor<tm1.1(CAG-cas9)Mabe>
Former Common nameCAG-Cas9
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Manabu Abe, Niigata University in 2022. C57BL/6N genetic background.
Strain descriptionCAG-Cas9 knockin mice at the ROSA26 locus.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-cas9)Mabe> targeted mutation 1.1, Manabu Abe
WPRE Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE
cas9 CRISPR associated protein 96cas9 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxP phage P1 loxP6loxP
chicken beta globin polyA6

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP site, Streptococcus pyogenes hspCas9, Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE), chicken beta globin polyA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of a application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorManabu Abe (Niigata University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data