Strain Data Sheet

RBRC11590

Strain Information

Image
BRC No.RBRC11590
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Cyfip2<tm1c(EUCOMM)Wtsi>/Tfur
Former Common nameCyfip2 flox
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeposited by Tkaahisa Furukawa in 2022. JM8A3.N1 (ES Cell) derived from C57BL6/N. Mice were furhter backcrossed to C57BL/6J for several genetations.
Strain descriptionCyfip2 floxed mice.
Colony maintenance
ReferencesHum Mol Genet Sep 11(2021) ;ddab268. doi: 10.1093/hmg/ddab268. 34508581

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cyfip2cytoplasmic FMR1 interacting protein 211Cyfip2tm1c(EUCOMM)Wtsi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Ordering Information

Donor DNAyeast FRT (flipase recombination target) site, phage P1 loxP sites, mouse Cyfip2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum Mol Genet. 2021 Sep 11;ddab268. doi: 10.1093/hmg/ddab268.The RECIPIENT must contact the DEPOSITOR and contract an MTA with Osaka Universtiy. The RECIPIENT shall acknowledge the EUCOMM as the Provider of the ES cells to generate KO mice.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data