Strain Data Sheet

RBRC11591

Strain Information

Image
BRC No.RBRC11591
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Peli3<em1Tfur>
Former Common nameC57BL/6-Peli3<em1Tfur>, Peli3 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain descriptionPeli3 mutant mice with 16 bp deletion at Exon 2.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Peli3
MGI:1924963		pellino 319Peli3<em1Tfur> endonuclease-mediated mutation 1, Takahisa Furukawa

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes U6 promoter, Streptococcus pyogenes gRNA scaffold, human U6 terminator, CMV, chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized)*, bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR)*, E. coli pUC origin* Not detected by PCR. Other introduced genes were not tested.
Research application
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR and contract an MTA with Osaka Universtiy.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data