Strain Data Sheet

RBRC11596

Strain Information

Image
BRC No.RBRC11596
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6;129Ola-Ddc<em1(hKO1)Nkn>
Former Common nameDdc-hKO1
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinpei Yamaguchi and Sheng Kit-Yeng, and deposited by Toru Nakano, Osaka University in 2022.G4 ES cells derived from 129 and B6N were used to generate this strain. Mixed genetic background.
Strain descriptionhKO1 knockin mice at the stop codon of the Ddc gene via P2a peptide.
Colony maintenance
References
https://doi.org/10.21203/rs.3.rs-296465/v1

Health Report

Examination Date / Room / Rack2023/05/08Room:2-検Rack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bsd Aspergillus terreus blasticidin resistance gene (Bsd)11Bsd mouse phosphoglycerate kinase promoter (PGK promoter)
Ddc
MGI:94876		dopa decarboxylase11Ddc<em1(hKO1)Nkn> endonuclease mediated mutation 1,
  • aromatic L-amino acid decarboxylase deficiency(MedGEN)
    • P2A porcine teschovirus-1 2A peptide11P2A
    hKO1 Humanized Kusabira-Orange (Verrillofungia concinna)11hKO1
    loxP phage P1 loxP11loxP
    loxP phage P1 loxP11loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAporcine teschovirus-1 2A peptide, Humanized Kusabira-Orange (Verrillofungia concinna)1 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus blasticidin resistance gene (Bsd), mouse Ddc genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorToru Nakano (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data