Strain Data Sheet

RBRC11596

Strain Information

Image
BRC No.RBRC11596
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6;129Ola-Ddc<em1(hKO1)Nkn>
Former Common nameDdc-hKO1
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinpei Yamaguchi and Sheng Kit-Yeng, and deposited by Toru Nakano, Osaka University in 2022.G4 ES cells derived from 129 and B6N were used to generate this strain. Mixed genetic background.
Strain descriptionhKO1 knockin mice at the stop codon of the Ddc gene via P2a peptide.
Colony maintenance
Referenceshttps://doi.org/10.21203/rs.3.rs-296465/v1

Health Report

Examination Date / Room / Rack2023/05/08Room:2-検Rack:C
2023/02/06Room:2-検Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ddcdopa decarboxylase11Ddc<em1(hKO1)Nkn>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BsdAspergillus terreus blasticidin resistance gene (Bsd)11Bsdmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide11P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
hKO1Humanized Kusabira-Orange (Verrillofungia concinna)11hKO1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Ordering Information

Donor DNAporcine teschovirus-1 2A peptide, Humanized Kusabira-Orange (Verrillofungia concinna)1 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus blasticidin resistance gene (Bsd), mouse Ddc genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorToru Nakano (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data