Strain Data Sheet

RBRC11597

Strain Information

Image
BRC No.RBRC11597
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(TH-GFP-IR84a/IR8a)2-1Koba
Former Common nameTH-GFP-IR84a/IR8a
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Kazuto Kobayashi, Fukushima Medical University in 2022. This strain was generated using fertilized eggs derived from (C57BL/6 x DBA/2)F1 and then mice were further backcrossed to C57BL/6J for 16 generations. C57BL/6J congenic strain.
Strain descriptionTransgenic mice expressing GFP-IR84a/IR8a under the control of rat TH promoter.
Colony maintenance
ReferencesJ. Neurosci., 40(43):8367-8385 (2020). 32994339

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IR84aIr84a (Ionotropic receptor 84a)(Drosophila melanogaster)UNIR84a

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IR8aIr8a (Ionotropic receptor 8a)(Drosophila melanogaster)UNIR8a

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)UNT2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Ordering Information

Donor DNAラット チロシン水酸化酵素(TH)プロモーター (ゲノムDNA), ウサギ betaグロビンイントロン(ゲノムDNA), ヒト カルレティキュリンシグナル配列(cDNA), クラゲ 緑色蛍光タンパク質(GFP) (cDNA), ショウジョウバエ イオンチャネル型受容体(IR84a)(cDNA) イオンチャネル型受容体(IR8a)(cDNA), 昆虫ウイルス(Thosea asigna virus) 2A配列(cDNA), サルウィルス(SV40) 初期遺伝子ポリA付加シグナル配列 (ゲノムDNA)
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 40(43):8367-8385 (2020).
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data