Strain Data Sheet
RBRC11597
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11597 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6.Cg-Tg(TH-GFP-IR84a/IR8a)2-1Koba |
| Former Common name | TH-GFP-IR84a/IR8a |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Kazuto Kobayashi, Fukushima Medical University in 2022. This strain was generated using fertilized eggs derived from (C57BL/6 x DBA/2)F1 and then mice were further backcrossed to C57BL/6J for 16 generations. C57BL/6J congenic strain. |
| Strain description | Transgenic mice expressing GFP-IR84a/IR8a under the control of rat TH promoter. |
| Colony maintenance | |
| References | Enhanced Retrieval of Taste Associative Memory by Chemogenetic Activation of Locus Coeruleus Norepinephrine Neurons. Fukabori R, Iguchi Y, Kato S, Takahashi K, Eifuku S, Tsuji S, Hazama A, Uchigashima M, Watanabe M, Mizuma H, Cui Y, Onoe H, Hikishima K, Yasoshima Y, Osanai M, Inagaki R, Fukunaga K, Nishijo T, Momiyama T, Benton R, Kobayashi K J. Neurosci., 40(43):8367-8385 (2020). 32994339Enhanced Retrieval of Taste Associative Memory by Chemogenetic Activation of Locus Coeruleus Norepinephrine Neurons. Ryoji Fukabori, Yoshio Iguchi, Shigeki Kato, Kazumi Takahashi, Satoshi Eifuku, Shingo Tsuji, Akihiro Hazama, Motokazu Uchigashima, Masahiko Watanabe, Hiroshi Mizuma, Yilong Cui, Hirotaka Onoe, Keigo Hikishima, Yasunobu Yasoshima, Makoto Osanai, Ryo Inagaki, Kohji Fukunaga, Takuma Nishijo, Toshihiko Momiyama, Richard Benton, Kazuto Kobayashi J. Neurosci., 40(43):8367-8385 (2020). 32994339 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | ||||
| IR84a | Ir84a (Ionotropic receptor 84a)(Drosophila melanogaster) | UN | IR84a | ||||
| IR8a | Ir8a (Ionotropic receptor 8a)(Drosophila melanogaster) | UN | IR8a | ||||
| T2A | Thosea asigna virus 2A peptide (insect) | UN | T2A | ||||
| SV40 polyA | UN | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | ラット チロシン水酸化酵素(TH)プロモーター (ゲノムDNA), ウサギ betaグロビンイントロン（ゲノムDNA）, ヒト カルレティキュリンシグナル配列（cDNA）, クラゲ 緑色蛍光タンパク質(GFP) (cDNA), ショウジョウバエ イオンチャネル型受容体(IR84a)(cDNA) イオンチャネル型受容体(IR8a)(cDNA), 昆虫ウイルス(Thosea asigna virus) 2A配列（cDNA）, サルウィルス（SV40） 初期遺伝子ポリA付加シグナル配列 (ゲノムDNA) |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 40(43):8367-8385 (2020). |
| Depositor | Kazuto Kobayashi (Fukushima Medical University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |