Strain Data Sheet
RBRC11604
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11604 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6-Spic<tm1.1Ksho> |
| Former Common name | Spi-C flox |
| H-2 Haplotype | |
| ES Cell line | JM8A3.N1 [C57BL/6N-A<tm1Brd>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2005. The neomycin resistance gene was deleted and backrossed to C57BL/6 mice by Wataru Ise, Osaka University Immunology Frontier Research Center (IFReC), around 2011-2013. |
| Strain description | Spi-C is an Ets family transcription factor. In Spi-C flox mice, two loxP sites are inserted in the introns of the Spi-C gene locus. Expression of cre recombinase leads to deletion of Spi-C coding exon(s) flanked by the loxP sites and disruption of the Spi-C gene locus. Spi-C-deficient mice show various phenotypes, such as ablation of splenic red pulp macrophage and defects in intestinal macrophage functions. |
| Colony maintenance | |
| References | Proc. Natl. Acad. Sci. USA, 115:8418-8423 (2018). 30061415 |
Health Report | |
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| Examination Date / Room / Rack | 2024/03/11Room:3-2Rack:C 2023/12/11Room:3-2Rack:C 2023/09/11Room:3-2Rack:C 2023/06/13Room:3-2Rack:C 2023/03/13Room:3-2Rack:C |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SpicSpi-C transcription factor (Spi-1/PU.1 related)10Spic<tm1.1Ksho>targeted mutation 1.1, Tsuneyasu Kaisho Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site10Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP |
Ordering Information | |
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| Donor DNA | Phage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Spic genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 115:8418-8423 (2018).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Tsuneyasu Kaisho) and CO-DEVELOPER (Dr. Wataru Ise) using the Approval Form (form D). (Contact info: Tsuneyasu Kaisho <tkaisho@wakayama-med.ac.jp>) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
| Depositor | Tsuneyasu Kaisho (RIKEN) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |