Strain Data Sheet

RBRC11604

Strain Information

Image
BRC No.RBRC11604
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6-Spic<tm1.1Ksho>
Former Common nameSpi-C flox
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2005. The neomycin resistance gene was deleted and backrossed to C57BL/6 mice by Wataru Ise, Osaka University Immunology Frontier Research Center (IFReC), around 2011-2013.
Strain descriptionSpi-C is an Ets family transcription factor. In Spi-C flox mice, two loxP sites are inserted in the introns of the Spi-C gene locus. Expression of cre recombinase leads to deletion of Spi-C coding exon(s) flanked by the loxP sites and disruption of the Spi-C gene locus. Spi-C-deficient mice show various phenotypes, such as ablation of splenic red pulp macrophage and defects in intestinal macrophage functions.
Colony maintenance
References
Heme ameliorates dextran sodium sulfate-induced colitis through providing intestinal macrophages with noninflammatory profiles.
Hisako Kayama, Masako Kohyama, Daisuke Okuzaki, Daisuke Motooka, Soumik Barman, Ryu Okumura, Masato Muneta, Katsuaki Hoshino, Izumi Sasaki, Wataru Ise, Hiroshi Matsuno, Junichi Nishimura, Tomohiro Kurosaki, Shota Nakamura, Hisashi Arase, Tsuneyasu Kaisho, Kiyoshi Takeda
Proc. Natl. Acad. Sci. USA, 115:8418-8423 (2018). 30061415

Health Report

Examination Date / Room / Rack2024/09/09Room:3-2Rack:CSentinel mouse program
2024/06/10Room:3-2Rack:CSentinel mouse program
2024/03/11Room:3-2Rack:CSentinel mouse program
2023/12/11Room:3-2Rack:CSentinel mouse program
2023/09/11Room:3-2Rack:CSentinel mouse program
2023/06/13Room:3-2Rack:CSentinel mouse program
2023/03/13Room:3-2Rack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site10Frt
Spic
MGI:1341168
Spi-C transcription factor (Spi-1/PU.1 related)10Spic<tm1.1Ksho> targeted mutation 1.1, Tsuneyasu Kaisho
loxP phage P1 loxP10loxP
loxP phage P1 loxP10loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAPhage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Spic genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 115:8418-8423 (2018). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Tsuneyasu Kaisho) and CO-DEVELOPER (Dr. Wataru Ise) using the Approval Form (form D). (Contact info: Tsuneyasu Kaisho <tkaisho@wakayama-med.ac.jp>) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorTsuneyasu Kaisho (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data