Strain Data Sheet

RBRC11629

Strain Information

Image
BRC No.RBRC11629
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Kctd19<em2Osb>
Former Common nameKctd19<delta POZ/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionKctd19 knockout mouse generated by the CRISPR/Cas9. 2,172 bp deletion in the Kctd19 gene. Homozygous male mice are infertile.
Colony maintenance
References
KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice.
Oura S, Koyano T, Kodera C, Horisawa-Takada Y, Matsuyama M, Ishiguro K I, Ikawa M
PLoS Genet., 17(5):e1009412 (2021). 33961623

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Kctd19
MGI:3045294		potassium channel tetramerisation domain containing 198Kctd19<em2Osb> endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Kctd19 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 17(5):e1009412 (2021). doi: 10.1371/journal.pgen.1009412. eCollection 2021 May.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data