Strain Data Sheet
RBRC11630
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11630 |
| Type | CRISPR/Cas9 |
| Species | Mus musculus |
| Strain name | STOCK Zfp541<em1Osb> |
| Former Common name | Zfp541<-26118/wt> |
| H-2 Haplotype | |
| ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Mice were generated using EGR-G01 (ES cell) derived from (129S2 x B6Cr)F1-Tg(CAG/Acr-EGFP). Mice were further crossed to B6D2F1. Mixed genetic background. |
| Strain description | Zfp541 KO mouse generated by the CRISPR/Cas9. 26,118 bp deletion in the IZfp541 gene. Homozygou males are infertile. |
| Colony maintenance | |
| References | KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. Oura S, Koyano T, Kodera C, Horisawa-Takada Y, Matsuyama M, Ishiguro K I, Ikawa M PLoS Genet., 17(5):e1009412 (2021). 33961623Evaluation of the efficacy of creatine chemical exchange saturation transfer imaging in assessing testicular maturity. Sohei Kuribayashi, Shinichiro Fukuhara, Go Tsujimura, Takahiro Imanaka, Koichi Okada, Norichika Ueda, Kentaro Takezawa, Hiroshi Kiuchi, Shigeyoshi Saito, Yusuke Takahashi, Hidetaka Kioka, Seiya Oura, Keisuke Shimada, Masahito Ikawa, Norio Nonomura Reprod Med Biol., 22(1):e12507(2023). 36845001 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Zfp541 MGI:3647699 | zinc finger protein 541 | 7 | Zfp541<em1Osb> | endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | human hU6 promoterCMV CMV enhancer (CBh)chicken chicken beta-actin promoter (CBh)SV40 SV40 nuclear localization signalStreptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNAEscherichia coli ampicillin resistance geneStreptomyces alboniger puromycin resistant geneMouse a part of Zfp541 gene |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 17(5):e1009412 (2021). doi: 10.1371/journal.pgen.1009412. eCollection 2021 May.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
|
BRC mice in Publications |
|---|
| No Data |