Strain Data Sheet

RBRC11631

Strain Information

Image
BRC No.RBRC11631
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2.B6-Tg(Zp3-EGFP/Cd9)1Osb
Former Common nameZP3-EGFP/CD9
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from C57BL/6 were used to generated this strain. Mice were further backcrossed to BDF1 for more than 10 generations.
Strain descriptionTransgenic mice expressing EGFP/Cd9 under the control of mouse Zp3 promoter.
Colony maintenance
References
The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice.
Kenji Miyado, Keiichi Yoshida, Kazuo Yamagata, Keiichi Sakakibara, Masaru Okabe, Xiaobiao Wang, Kiyoko Miyamoto, Hidenori Akutsu, Takahiko Kondo, Yuji Takahashi, Tadanobu Ban, Chizuru Ito, Kiyotaka Toshimori, Akihiro Nakamura, Masahiko Ito, Mami Miyado, Eisuke Mekada, Akihiro Umezawa
Proc. Natl. Acad. Sci. USA, 105(35):12921-6(2008). 18728192

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAMouse Zp3 promoter, Jellyfish Enhanced green fluorescence protein, Mouse Cd9,Rabbit b-Globin pA signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA, 105(35):12921-6(2008). doi: 10.1073/pnas.0710608105. Epub 2008 Aug 26.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data