Strain Data Sheet
RBRC11637
Strain Information | |
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| Image | |
| BRC No. | RBRC11637 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6D2-Kctd19<em1Osb> Tg(Clgn-3xFLAG-Kctd19)1Osb Tg(Clgn-Kctd19/3xHA)2Osb |
| Former Common name | Kctd19<del/del>, Tg#2 (FLAG/HA) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from RBRC11037 were used to generate this line. Mixed genetic background. |
| Strain description | Compound strain harboring two transgenes expressing C-terminally tagged Kctd19 under the control of Clgn promoter (3xFLAG and 3xHA tags). This strain also has mutation in the Kctd19 gene. 9620 bp deletion in the Kctd19 gene. |
| Colony maintenance | |
| References | PLoS Genet., 17(5):e1009412 (2021). 33961623 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Kctd19potassium channel tetramerisation domain containing 198Kctd19<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAGmouse Clgn promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Kctd19potassium channel tetramerisation domain containing 19UNKctd19 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Kctd19potassium channel tetramerisation domain containing 19UNKctd19mouse Clgn promoter |
Ordering Information | |
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| Donor DNA | Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Kctd19 gene, Mouse calmegin promoter, Rabbit beta-globin polyadenylation signal Mouse Kctd19 cDNA, Influenza virus Influenza hemagglutinin (HA) tag, synthetic FLAG tag |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 17(5):e1009412 (2021). doi: 10.1371/journal.pgen.1009412. eCollection 2021 May.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |