Strain Data Sheet
RBRC11637
Strain Information | |
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| Image | |
| BRC No. | RBRC11637 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6D2-Kctd19<em1Osb> Tg(Clgn-3xFLAG-Kctd19)1Osb Tg(Clgn-Kctd19/3xHA)2Osb |
| Former Common name | Kctd19<del/del>, Tg#2 (FLAG/HA) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from RBRC11037 were used to generate this line. Mixed genetic background. |
| Strain description | Compound strain harboring two transgenes expressing C-terminally tagged Kctd19 under the control of Clgn promoter (3xFLAG and 3xHA tags). This strain also has mutation in the Kctd19 gene. 9620 bp deletion in the Kctd19 gene. |
| Colony maintenance | |
| References | KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. Oura S, Koyano T, Kodera C, Horisawa-Takada Y, Matsuyama M, Ishiguro K I, Ikawa M PLoS Genet., 17(5):e1009412 (2021). 33961623 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Kctd19 MGI:3045294 | potassium channel tetramerisation domain containing 19 | 8 | Kctd19<em1Osb> | endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
| FLAG | FLAG tag (synthetic) | UN | FLAG | mouse Clgn promoter | |||
| Kctd19 | potassium channel tetramerisation domain containing 19 | UN | Kctd19 | mouse Clgn promoter | |||
| Kctd19 | potassium channel tetramerisation domain containing 19 | UN | Kctd19 | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Kctd19 gene, Mouse calmegin promoter, Rabbit beta-globin polyadenylation signal Mouse Kctd19 cDNA, Influenza virus Influenza hemagglutinin (HA) tag, synthetic FLAG tag |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 17(5):e1009412 (2021). doi: 10.1371/journal.pgen.1009412. eCollection 2021 May.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |