Strain Data Sheet
RBRC11664
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11664 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6D2-Lrrc23<em1Osb> Tg(Clgn-Lrrc23/FLAG)1Osb Tg(Clgn-Lrrc23/1D4)1Osb |
| Former Common name | Lrrc23<-8398/wt>, Lrrc23-FLAG/1D4 Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from RBRC11481 were used to generate this line. Mixed genetic background. |
| Strain description | Compound strain harboring two transgenes expressing C-terminally tagged Lrrc23 under the control of Clgn promoter (3xFLAG and 1D4 tags). This strain also has mutation in the Lrrc23 gene. 8,398 bp deletion in the Lrrc23 gene. |
| Colony maintenance | |
| References | LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. Zhang X, Sun J, Lu Y, Zhang J, Shimada K, Noda T, Zhao S, Koyano T, Matsuyama M, Zhou S, Wu J, Ikawa M, Liu M J. Cell Sci., 134(20):jcs259381 (2021). 34585727 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Lrrc23 MGI:1315192 | leucine rich repeat containing 23 | 6 | Lrrc23<em1Osb> | endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
| 1D4 | 1D4 tag (synthetic) | UN | 1D4 | ||||
| FLAG | FLAG tag (synthetic) | UN | FLAG | ||||
| HBB2 | rabbit bata-globin polyA | UN | HBB2 | ||||
| Lrrc23 | leucine rich repeat containing 23 | UN | Lrrc23 | mouse Clgn promoter | |||
| Lrrc23 | leucine rich repeat containing 23 | UN | Lrrc23 | mouse Clgn promoter | |||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | Streptococcus pyogenes crRNA, tracrRNA, Mousea part of Lrrc23 gene, Mouse Calmegin promoter, Rabbit Beta-globin polyadenylation signal, Mouse Lrrc23 cDNA,Synthetic FLAG, 1D4 |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 134(20):jcs259381 (2021). doi: 10.1242/jcs.259381. Epub 2021 Oct 21.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
|
BRC mice in Publications |
|---|
| No Data |