Strain Data Sheet

RBRC11665

Strain Information

Image
BRC No.RBRC11665
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Mtl5<em1Osb> Tg(Clgn-Tesmin/BioID2/3xFLAG)1Osb
Former Common nameTesmin<-316/-316> BioID2 Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from RBRC09987 were used to generate this line. Mixed genetic background.
Strain descriptionCompound strain harboring a transgene expressing C-terminally tagged Tesmin under the control of Clgn promoter (BioID2/3xFLAG tags). This strain also has mutation in the Kctd19 gene. 316 bp deletion in the Tesmin gene.
Colony maintenance
ReferencesSci. Rep., 12(1):22198 (2022). 36564444

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tesmintestis expressed metallothionein like19Tesmin<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka UniversityMtl5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BioID2proximity-dependent biotin identification 2UNBioID2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tesmintestis expressed metallothionein likeUNTesminMtl5mouse Clgn promoter

Ordering Information

Donor DNAhuman hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Tesmin gene, mouse Clgn promoter, mouse Tesmin cDNA, synthetic Flag tag, Aquifex aeolicus Biotin ligase (BirA R40G), rabbit globin polyA signal
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 12(1):22198 (2022).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data