Strain Data Sheet
RBRC11676
Strain Information | |
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| Image | |
| BRC No. | RBRC11676 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129S-Fosb<tm1.1Yun> |
| Former Common name | Fosb<d> |
| H-2 Haplotype | |
| ES Cell line | CCE/EK.CCE [129S/SvEv-Gpi1<c>] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Yusaki Nakabeppu, Kyusyu University in 2022. This strain was generated using CCE/EK.CCE ES Cell derived from 129S/SvEv-Gpi1<c> and then mice were backcrossed to C57BL/6J for 46 generations. C57BL/6J congenic strain. |
| Strain description | A floxed neo cassette was inserted in intron 3 and the sequence encoding the alternative splicing donor site for the exonic intron in exon 4 was replaced with tandem stop codons (GTG, AGA to TAG, TGA) via homologous recombination. This prevents the expression of the short transcript but allows expression of the longer transcript that encodes two isoforms (deltaFosb and delta2deltaFosb). Cre-mediated recombination removed the neo cassette. |
| Colony maintenance | |
| References | Antagonistic regulation of cell-matrix adhesion by FosB and DeltaFosB/Delta2DeltaFosB encoded by alternatively spliced forms of fosB transcripts. Yoshinori N Ohnishi, Kunihiko Sakumi, Katsuhisa Yamazaki, Yoko H Ohnishi, Tomofumi Miura, Yohei Tominaga, Yusaku Nakabeppu Mol. Biol. Cell., 19(11):4717-29 (2008). 18753407FosB is essential for the enhancement of stress tolerance and antagonizes locomotor sensitization by ΔFosB. Yoshinori N Ohnishi, Yoko H Ohnishi, Masaaki Hokama, Hiroko Nomaru, Katsuhisa Yamazaki, Yohei Tominaga, Kunihiko Sakumi, Eric J Nestler, Yusaku Nakabeppu Biol. Psychiatry, 70(5):487-95 (2011). 21679928 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Fosb MGI:95575 | FBJ osteosarcoma oncogene B | 7 | Fosb<tm1.1Yun> MGI:5634956 | targeted mutation 1.1, Yusaku Nakabeppu | |||
| loxP | phage P1 loxP | 7 | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Bacteriophage P1 loxP |
| Research application | Cre/loxP system |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell., 19(11):4717-29 (2008). |
| Depositor | Yusaku Nakabeppu (Kyushu University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |