Strain Data Sheet

RBRC11677

update : 2025-02-20

Strain Information
Image
BRC No.	RBRC11677
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.129S-Fosb<tm2.1Yun>
Former Common name	Fosb<G>
H-2 Haplotype
ES Cell line	CCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain development	Deposited by Yusaki Nakabeppu, Kyusyu University in 2022. This strain was generated using CCE/EK.CCE ES Cell derived from 129S/SvEv-Gpi1<c> and then mice were backcrossed to C57BL/6J for 41 generations. C57BL/6J congenic strain.
Strain description	A destabilized variant of the enhanced green fluorescent protein (EGFP) was inserted in frame in exon 2 and the remainder of exon 2 and all of exon 3 were replaced by a floxed neo cassette via homologous recombination. Cre mediated recombination removed the neo cassette.
Colony maintenance
References	Antagonistic regulation of cell-matrix adhesion by FosB and DeltaFosB/Delta2DeltaFosB encoded by alternatively spliced forms of fosB transcripts. Yoshinori N Ohnishi, Kunihiko Sakumi, Katsuhisa Yamazaki, Yoko H Ohnishi, Tomofumi Miura, Yohei Tominaga, Yusaku Nakabeppu Mol. Biol. Cell., 19(11):4717-29 (2008). 18753407 fosB-null mice display impaired adult hippocampal neurogenesis and spontaneous epilepsy with depressive behavior. Noriko Yutsudo, Takashi Kamada, Kosuke Kajitani, Hiroko Nomaru, Atsuhisa Katogi, Yoko H Ohnishi, Yoshinori N Ohnishi, Kei-ichiro Takase, Kunihiko Sakumi, Hiroshi Shigeto, Yusaku Nakabeppu Neuropsychopharmacology, 38(5):895-906 (2013). 23303048

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Fosb MGI:95575	FBJ osteosarcoma oncogene B	7	Fosb<tm2.1Yun> MGI:5634957	targeted mutation 2.1, Yusaku Nakabeppu
d2EGFP	EGFP destabilized by residues 422-461 of mouse ornithinedecarboxylase	7	d2EGFP
loxP	phage P1 loxP	7	loxP

Phenotype
Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information
Donor DNA	Bacteriophage P1 loxP, Jellyfish/mouse d2EGFP (EGFP destabilized by residues 422-461 of mouse ornithinedecarboxylase)
Research application	Cre/loxP system Fluorescent Proteins/lacZ System
Specific Term and Conditions	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell., 19(11):4717-29 (2008).
Depositor	Yusaku Nakabeppu (Kyushu University)
Strain Status	Frozen sperm
Strain Availability	Recovery and QC required prior to distribution
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data