Strain Data Sheet

RBRC11678

update : 2025-02-20

Strain Information
Image
BRC No.	RBRC11678
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.129S-Fosb<tm3.1Yun>
Former Common name	Fosb<F>
H-2 Haplotype
ES Cell line	CCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain development	Deposited by Yusaki Nakabeppu, Kyusyu University in 2022. This strain was generated using CCE/EK.CCE ES Cell derived from 129S/SvEv-Gpi1<c> and then mice were backcrossed to C57BL/6J for 23 generations. C57BL/6J congenic strain.
Strain description	Three bases (GAGGTGAGA->GAAGTTCGA) in the alternative splicing donor site and two bases (AGTGAA->TCTGAA) in the splice acceptor site were modified via homologous recombination to prevent expression of the long transcript while retaining expression of the shorter transcript. A floxed neo cassette was also inserted in intron 3. Cre mediated recombination removed the neo cassette. This allele lacks expression of the deltaFosb isoform.
Colony maintenance
References	Antagonistic regulation of cell-matrix adhesion by FosB and DeltaFosB/Delta2DeltaFosB encoded by alternatively spliced forms of fosB transcripts. Yoshinori N Ohnishi, Kunihiko Sakumi, Katsuhisa Yamazaki, Yoko H Ohnishi, Tomofumi Miura, Yohei Tominaga, Yusaku Nakabeppu Mol. Biol. Cell., 19(11):4717-29 (2008). 18753407 Fosb gene products contribute to excitotoxic microglial activation by regulating the expression of complement C5a receptors in microglia. Hiroko Nomaru, Kunihiko Sakumi, Atsuhisa Katogi, Yoshinori N Ohnishi, Kosuke Kajitani, Daisuke Tsuchimoto, Eric J Nestler, Yusaku Nakabeppu Glia, 62(8):1284-98 (2014). 24771617

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Fosb MGI:95575	FBJ osteosarcoma oncogene B	7	Fosb<tm3.1Yun> MGI:5634958	targeted mutation 3.1, Yusaku Nakabeppu
loxP	phage P1 loxP	7	loxP

Phenotype
Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information
Donor DNA	Bacteriophage P1 loxP
Research application	Cre/loxP system
Specific Term and Conditions	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell., 19(11):4717-29 (2008).
Depositor	Yusaku Nakabeppu (Kyushu University)
Strain Status	Frozen sperm
Strain Availability	Recovery and QC required prior to distribution
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data