Strain Data Sheet

RBRC11686

Strain Information

Image
BRC No.RBRC11686
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6;Cg-Oxtr<em1(1xPA/T2A/tdTomato)Yinn>
Former Common nameOxtr<1xPA-tdTomato>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Yukiko U. Inoue and Takayoshi Inoue, National Center of Neurology and Psychiatry in 2022. This strain was generated using fertilized eggs derived from (C57BL/6 x C3H)F1. Mixed genetic background.
Strain descriptiontdTomato knockin mice via 1xPA/T2A at the C-terminus of the Oxtr gene. Homozygous mice are fertile.
Colony maintenance
References
Targeting Neurons with Functional Oxytocin Receptors: A Novel Set of Simple Knock-In Mouse Lines for Oxytocin Receptor Visualization and Manipulation.
Yukiko U Inoue, Hideki Miwa, Kei Hori, Ryosuke Kaneko, Yuki Morimoto, Eriko Koike, Junko Asami, Satoshi Kamijo, Mitsuhiko Yamada, Mikio Hoshino, Takayoshi Inoue
eNeuro, 9(1):ENEURO.0423-21.2022 (2022). 35082173

Health Report

Examination Date / Room / Rack2024/08/26Room:4-CRack:DSentinel mouse program
2024/05/27Room:4-CRack:DSentinel mouse program
2024/02/26Room:4-CRack:DSentinel mouse program
2023/11/27Room:4-CRack:DSentinel mouse program
2023/08/28Room:4-CRack:DSentinel mouse program
2023/05/29Room:4-CRack:DSentinel mouse program
2023/02/27Room:4-CRack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Oxtr
MGI:109147
oxytocin receptor6Oxtr<em1(1xPA/T2A/tdTomato)Yinn> endonuclease-mediated mutation 1, Yukiko U Inoue
PA PA tag (GVAMPGAEDDVV) (synthetic) human Podoplanin6PA
T2A Thosea asigna virus 2A peptide (insect)6T2A
tdTomato tandem dimeric Red Fluorescent Protein (Discosoma sp.)6tdTomato

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman Podoplanin PA-tag sequence, Thosea asigna virus 2A peptide (insect), Discosoma sp. tandem dimeric Red Fluorescent Protein (tdTomato) cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. eNeuro. Feb 15;9(1):ENEURO.0423-21.2022 (2022) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakayoshi Inoue (National Center of Neurology and Psychiatry)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (TBUSAMTA_Nonprofit_BROAD (non-US)) / (TBUSA_Broad_lisence (US))
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Sep 2023

BRC mice in Publications

No Data