Strain Data Sheet

RBRC11727

Strain Information

Image
BRC No.RBRC11727
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6J.B6N-Islr<tm1b(EUCOMM)Wtsi>/Ea
Former Common nameMeflin-KO
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesCancer Res., 79(20):5367-5381 (2019). 31439548
Genomics, 44: 273-279 (1997). 9325048
Sci. Rep., 6:22288 (2016). 26924503
Circ. Res., 25(4):414-430 (2019). 31221024

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Islrimmunoglobulin superfamily containing leucine-rich repeat9Islr<tm1b(EUCOMM)Wtsi>targeted mutation 1b, Wellcome Trust Sanger InstituteMeflin

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)9En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Ordering Information

Donor DNASaccharomyces cerevisiae yeast FRT (flippase recombination target) site, Mouse En2 intron 2/exon 3 splice acceptor sequence (En2 SA), EMCV internal ribosomal entry site (IRES), E. coli lacZ, Phage P1 loxP site, mouse Islr genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 6:22288 (2016).　Cancer Res., 79(20):5367-5381 (2019). Circ. Res., 125(4):414-430 (2019).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is restricted to a RECIPIENT of a not-for profit organization for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT shall acknowledge the EUCOMM as the Provider of the ES cells to generate KO mice.
DepositorAtsushi Enomoto (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data