Strain Information | |
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Image | |
BRC No. | RBRC11727 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6J.B6N-Islr<tm1b(EUCOMM)Wtsi>/Ea |
Former Common name | Meflin-KO |
H-2 Haplotype | |
ES Cell line | JM8A3.N1 [C57BL/6N-A<tm1Brd>] |
Background strain | |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References | Cloning of the cDNA for a new member of the immunoglobulin superfamily (ISLR) containing leucine-rich repeat (LRR). Nagasawa A, Kubota R, Imamura Y, Nagamine K, Wang Y, Asakawa S, Kudoh J, Minoshima S, Mashima Y, Oguchi Y, Shimizu N Genomics, 44: 273-279 (1997). 9325048Identification of Meflin as a Potential Marker for Mesenchymal Stromal Cells. Maeda K, Enomoto A, Hara A, Asai N, Kobayashi T, Horinouchi A, Maruyama S, Ishikawa Y, Nishiyama T, Kiyoi H, Kato T, Ando K, Weng L, Mii S, Asai M, Mizutani Y, Watanabe O, Hirooka Y, Goto H, Takahashi M Sci. Rep., 6:22288 (2016). 26924503Roles of the Mesenchymal Stromal/Stem Cell Marker Meflin in Cardiac Tissue Repair and the Development of Diastolic Dysfunction. Hara A, Kobayashi H, Asai N, Saito S, Higuchi T, Kato K, Okumura T, Bando Y K, Takefuji M, Mizutani Y, Miyai Y, Saito S, Maruyama S, Maeda K, Ouchi N, Nagasaka A, Miyata T, Mii S, Kioka N, Worthley D L, Murohara T, Takahashi M, Enomoto A Circ. Res., 25(4):414-430 (2019). 31221024Meflin-Positive Cancer-Associated Fibroblasts Inhibit Pancreatic Carcinogenesis. Mizutani Y, Kobayashi H, Iida T, Asai N, Masamune A, Hara A, Esaki N, Ushida K, Mii S, Shiraki Y, Ando K, Weng L, Ishihara S, Ponik S M, Conklin M W, Haga H, Nagasaka A, Miyata T, Matsuyama M, Kobayashi T, Fujii T, Yamada S, Yamaguchi J, Wang T, Woods S L, Worthley D, Shimamura T, Fujishiro M, Hirooka Y, Enomoto A, Takahashi M Cancer Res., 79(20):5367-5381 (2019). 31439548 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
En2 | En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence) | 9 | En2 | ||||
Frt | yeast FRT (flippase recombination target) site | 9 | Frt | ||||
Ires | internal ribosomal entry site (EMCV) | 9 | |||||
Islr MGI:1349645 | immunoglobulin superfamily containing leucine-rich repeat | 9 | Islr<tm1b(EUCOMM)Wtsi> MGI:6119548 | targeted mutation 1b, Wellcome Trust Sanger Institute | |||
lacZ | beta-galactosidase (E. coli) | 9 | |||||
loxP | phage P1 loxP | 9 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Saccharomyces cerevisiae yeast FRT (flippase recombination target) site, Mouse En2 intron 2/exon 3 splice acceptor sequence (En2 SA), EMCV internal ribosomal entry site (IRES), E. coli lacZ, Phage P1 loxP site, mouse Islr genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 6:22288 (2016). Cancer Res., 79(20):5367-5381 (2019). Circ. Res., 125(4):414-430 (2019).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is restricted to a RECIPIENT of a not-for profit organization for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT shall acknowledge the EUCOMM as the Provider of the ES cells to generate KO mice. |
Depositor | Atsushi Enomoto (Nagoya University) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |