Strain Data Sheet
RBRC11734
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11734 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6-Tg(Nodal NDE-5HT6/GCaMP6,RGECO1)130AHmd |
| Former Common name | NDE4-hsp-5HT6GCaMP6-2A-RGECO1(130A) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry. Katsutoshi Mizuno, Kei Shiozawa, Takanobu A Katoh, Katsura Minegishi, Takahiro Ide, Yayoi Ikawa, Hiromi Nishimura, Katsuyoshi Takaoka, Takeshi Itabashi, Atsuko H Iwane, Junichi Nakai, Hidetaka Shiratori, Hiroshi Hamada Sci. Adv., 6(30):eaba1195 (2020). 32743070 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| GCaMP6 | RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA | UN | GCaMP6 | ||||
| P2A | porcine teschovirus-1 2A peptide | UN | P2A | ||||
| RGECO1 | red fluorescent protein (Discosoma sp.) | UN | RGECO1 | ||||
| SV40 polyA | UN | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | オワンクラゲ 蛍光タンパク質遺伝子（GFP誘導体GCaMP6）, 造礁ｻﾝｺﾞ(ｲｿｷﾞﾝﾁｬｸﾓﾄﾞｷ) DsRed 誘導体 RGECO1, マウス Nodal 遺伝子エンハンサー配列(NDE), 発現調節用プロモーター配列(Hsp68), セロトニンレセプター遺伝子 (5-HT6), porcine teschovirus-1 ﾎﾟﾘﾍﾟﾌﾟﾁﾄﾞ自己切断配列（2A cleavage site), SV40 ポリA付加配列 |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Adv., 6(30):eaba1195 (2020). doi: 10.1126/sciadv.aba1195. |
| Depositor | Hiroshi Hamada (RIKEN BDR) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |