Strain Data Sheet
RBRC11878
Strain Information | |
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| Image | |
| BRC No. | RBRC11878 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129-Slitrk2<tm1.1Jaru> |
| Former Common name | Slitrk2 knockout mouse |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Dr. Jun Aruga, RIKEN Brain Science Institute (2005). Mice with germline transmission were first mated with mice transgenic for Cre recombinase under the control of the cytomegalovirus immediate early enhancerchicken b-actin hybrid (CAG) promotor. Mice carrying the mutated Slitrk2 allele were backcrossed to C57BL/6J for more than eight generations. Slitrk2 knockout (KO) mice exhibited increased locomotor activity in novel environments, antidepressant-like behaviors, enhanced vestibular function, and increased plasticity at mossy fiber–CA3 synapses with reduced sensitivity to serotonin. A serotonin metabolite was increased in the hippocampus and amygdala, and serotonergic neurons in the raphe nuclei were decreased in Slitrk2 KO mice. When Slitrk2 KO mice were treated with methylphenidate, lithium, or fluoxetine, the mood stabilizer lithium showed a genotype-dependent effect. Slitrk2 KO mice are useful for studies of neuropsychiatric disorders. |
| Strain description | Mutation details: A targeting vector was designed to delete a 5.8 kb region containing entire open-reading frame. A floxed neo replaced the sequence and was subsequently excised by crossing with mice expressing Cre in the germ line. Western blot of mutant brain samples showed no bands corresponding to Slitrk2 protein. |
| Colony maintenance | |
| References | Slitrk2 deficiency causes hyperactivity with altered vestibular function and serotonergic dysregulation. Kei-Ichi Katayama, Naoko Morimura, Katsunori Kobayashi, Danielle Corbett, Takehito Okamoto, Veravej G Ornthanalai, Hayato Matsunaga, Wakako Fujita, Yoshifumi Matsumoto, Takumi Akagi, Tsutomu Hashikawa, Kazuyuki Yamada, Niall P Murphy, Soichi Nagao, Jun Aruga iScience, 25(7):104604 (2022). 35789858 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Slitrk2 MGI:2679449 | SLIT and NTRK-like family, member 2 | X | Slitrk2<tm1.1Jaru> | targeted mutation 1.1, Jun Aruga | |||
| loxP | phage P1 loxP | X | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Bacteriophage P1 loxP site, mouse Slitrk2 genomic DNA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. iScience. Jun 14;25(7):104604.(2022) doi: 10.1016/j.isci.2022.104604 |
| Depositor | Jun Aruga (Nagasaki University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |