Strain Data Sheet

RBRC11885

Strain Information

Image
BRC No.RBRC11885
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6N.129(Cg)-Gt(ROSA)26Sor<tm1(CAG-loxP-Stop-loxP-OsTIR1F74G,-EGFP)Tani>
Former Common nameOsTIR1 Tg
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2025/01/07Room:3-BRack:ISentinel mouse program
2024/10/07Room:3-BRack:ISentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP
Frt yeast FRT (flippase recombination target) site6Frt
Frt yeast FRT (flippase recombination target) site6Frt
GH Growth hormone polyA (Bovine)6GH
Gt(ROSA)26Sor
MGI:104735
gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-loxP-Stop-loxP-OsTIR1F7,-EGFP)Tani> targeted mutation 1, Ichiro Taniuchi
Ires internal ribosomal entry site (EMCV)6
OsTIR1 F74G Oryza sativa OsTIR1 F74G6OsTIR1 F74G CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
SA Splice acceptor (adenovirus)6SA
STOP 6STOP
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAAdenovirus splice acceptor, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Enterobacteria phage P1 LoxP sites, E.coli neomycin resistance gene, SV40 polyA, HSV Tk polyA, Oryza sativa OsTIR1 F74G cDNA, Sachcharomyces cerevisiae FRT sites, EMCV IRES (internal ribosomal entry site), Aequorea victoria EGFP cDNA, bovine BGH polyA, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) (2) For use of the BIOLOGICAL RESOURCE by a for-profit organization or by a not-for profit organization for a for-profit research, the RECIPIENT must reach agreement on Terms and Conditions of use of it with the DEPOSITOR and must obtain a prior written consist from the DEPOSITOR. (3) RECIPIENT must contact the DEPOSITOR in case of application for any patents or for-profit use based o the results from the use of the BIOLOGICAL RESOURCE.
DepositorIchiro Taniuchi (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data