Strain Data Sheet
RBRC11898
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11898 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Aldoc<tm1(Venus)Ksak>/Isugi |
| Former Common name | Aldoc<tm1Isugi>, Aldoc<Venus/Venus>, Aldoc-Venus:C7BL/6N, Alsoc-Venus knock-in mice, MGI ID: 5620954 |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | The Aldoc-Venus mouse strain was proposed by Izumi Sugihara and produced by Kenji Sakimura’s Laboratory in Niigata University in 2009-2010. The generation of the Aldoc-Venus mice was supported by a Grant-in-Aid for Scientific Research on Innovative Areas (Comprehensive Brain Science Network) from the Ministry of Education, Science, Sports and Culture of Japan. The stain can be maintained by mating between homozygotes. No apparent phenotypic features except for the venus expression in the brain. |
| Strain description | A gene of mutated green fluorescent protein (Venus) is inserted to the exon 2 of the Aldoc gene in Aldoc-Venus mice. In this mouse strain, the knocked in Venus gene is expressed under the same transcriptional control as the Aldoc gene. Consequently, zebrin (aldolase C, Aldoc)- positive Purkinje cells, which are arranged in the longitudinal striped pattenrn in the cerebellum, are visualized by Venus expression in Aldoc-Venus mice. Additionally astrocytes in the cebtral nervous system are mildly visualuzed by week Venus expression. Many retinal neurons are also visualuzed by Venus expression. |
| Colony maintenance | |
| References | Detailed expression pattern of aldolase C (Aldoc) in the cerebellum, retina and other areas of the CNS studied in Aldoc-Venus knock-in mice. Hirofumi Fujita, Hanako Aoki, Itsuki Ajioka, Maya Yamazaki, Manabu Abe, Arata Oh-Nishi, Kenji Sakimura, Izumi Sugihara PLoS One, 9(1):e86679 (2014). 24475166J. Comp. Neurol., 532(1)e25581(2024). 38289187 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Aldoc MGI:101863 | aldolase C, fructose-bisphosphate | 11 | Aldoc<tm1(Venus)Ksak> | targeted mutation 1, Kenji Sakimura | |||
| Venus | Yellow Fluorescent Protein (Aequorea victoria) | 11 | Venus | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | マウス マウス由来 PGK 遺伝子 promoter, 大腸菌 大腸菌由来 Neo 耐性遺伝子, マウス マウス由来 PGK poly(A)シグナル, 出芽酵母 フリッパーゼ認識標的部位配列 FRT, オワンクラゲ オワンクラゲ Venus(EGFP の変異体)遺伝子, phage T7 gb2 promoter, |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 9(1):e86679 (2014). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Izumi Sugihara (Tokyo Medical and Dental University) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |