Strain Data Sheet
RBRC11939
Strain Information | |
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| Image | |
| BRC No. | RBRC11939 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Gt(ROSA)26Sor<tm2(CAG-MFG-E8<EPT>)Ksawa |
| Former Common name | R26-EPT |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Synaptic pruning of murine adult-born neurons by microglia depends on phosphatidylserine. Chihiro Kurematsu, Masato Sawada, Masaki Ohmuraya, Motoki Tanaka, Kazuya Kuboyama, Takashi Ogino, Mami Matsumoto, Hisashi Oishi, Hiroyuki Inada, Yuri Ishido, Yukina Sakakibara, Huy Bang Nguyen, Truc Quynh Thai, Shinichi Kohsaka, Nobuhiko Ohno, Maki K Yamada, Masato Asai, Masahiro Sokabe, Junichi Nabekura, Kenichi Asano, Masato Tanaka, Kazunobu Sawamoto J. Exp. Med., 219(4):e20202304 (2022). 35297954 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 6 | EGFP | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm2(CAG-MFG–E8<EPT>)Ksawa> | targeted mutation 2, Kazunobu Sawamoto | |||
| Ires | internal ribosomal entry site (EMCV) | 6 | |||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 6 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | ||||
| MFG–E8<EPT> | 6 | ||||||
| SV40 polyA | 6 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | chicken, rabbit, CMV(クラス 1,2) CAG promoter, バクテリオファージ(クラス1)loxP, 大腸菌（クラス 1）ネオマイシン耐性遺伝子, SV40(クラス 2) STOP配列,酵母（クラス 1） ＦＲＴ, Encephalomyocarditis (EMC) Virus(クラス 2) ＩＲＥＳJellyfish(クラス 1) EGFP, SV40(クラス 2) ポリA, マウス（クラス 1） MFG-E8(EPT) |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Exp Med. Apr 4;219(4):e20202304.(2022)Prior to publish the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR. Do not supply the BIOLOGICAL RESOURCE to third parties without the DEPOSITOR' s permission. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results of BIOLOGICAL RESOURCE. |
| Depositor | Kazunobu Sawamoto (Nagoya City University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |