Strain Data Sheet

RBRC12081

Strain Information

Image
BRC No.RBRC12081
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Myh7<em1Smur>
Former Common nameGFP-Myh7
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Difference in potential DNA methylation impact on gene expression between fast- and slow-type myofibers.
Mika Oe, Koichi Ojima, Susumu Muroya
Physiol. Genomics, 53(2):69-83 (2021). 33459151

Endogenous slow and fast myosin dynamics in myofibers isolated from mice expressing GFP-Myh7 and Kusabira Orange-Myh1.
Koichi Ojima, Masahiro Kigaki, Emi Ichimura, Takahiro Suzuki, Ken Kobayashi, Susumu Muroya, Takanori Nishimura
Am. J. Physiol. Cell Physiol., 323(2):C520-C535 (2022). 35759444

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)14EGFP
Myh7
MGI:2155600		myosin, heavy polypeptide 7, cardiac muscle, beta14Myh7<em1Smur>
MGI:7411131		endonuclease-mediated mutation 1, Susumu Muroya
  • congenital fiber-type disproportion myopathy(MedGEN)

  • congenital myopathy 7A, myosin storage, autosomal dominant(MedGEN)

  • dilated cardiomyopathy 1S(MedGEN)
    • more 5 Diseases
  • hypertrophic cardiomyopathy 1(MedGEN)

  • hypertrophic cardiomyopathy(DisGeNET)

  • MYH7-related skeletal myopathy(DisGeNET, MedGEN)

  • myopathy, myosin storage, autosomal recessive(MedGEN)

  • obsolete myopathy, myosin storage, autosomal dominant(MedGEN)
    • SV40 polyA14

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAjellyfish EGFP cDNA, SV40 pA, E. coli ColE1 ori, mouse Myh7 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Physiol. Genomics, 53(2):69-83 (2021). Am. J. Physiol. Cell. Physiol., 323(2):C520-C535 (2022). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKoichi Ojima (National Agriculture and Food Research Organization)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data