Strain Data Sheet

RBRC12082

Strain Information

Image
BRC No.RBRC12082
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Myh1<em1Tnsh>
Former Common nameKuO-Myh1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Difference in potential DNA methylation impact on gene expression between fast- and slow-type myofibers.
Mika Oe, Koichi Ojima, Susumu Muroya
Physiol. Genomics, 53(2):69-83 (2021). 33459151

Endogenous slow and fast myosin dynamics in myofibers isolated from mice expressing GFP-Myh7 and Kusabira Orange-Myh1.
Koichi Ojima, Masahiro Kigaki, Emi Ichimura, Takahiro Suzuki, Ken Kobayashi, Susumu Muroya, Takanori Nishimura
Am. J. Physiol. Cell Physiol., 323(2):C520-C535 (2022). 35759444

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
KO Kusabira Orange (Fungia concinna)11KO
Myh1
MGI:1339711		myosin, heavy polypeptide 1, skeletal muscle, adult11Myh1<em1Tnsh>
MGI:7411129		endonuclease-mediated mutation 1, Takanori Nishimura

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAFungia concinna Kusabira Orange cDNA, mouse Myh1 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Physiol. Genomics, 53(2):69-83 (2021). Am. J. Physiol. Cell. Physiol., 323(2):C520-C535 (2022). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKoichi Ojima (National Agriculture and Food Research Organization)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data