Strain Data Sheet
RBRC12094
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC12094 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6-Il2<em1(mCherry)Schik> |
| Former Common name | Il2-mCherry |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | A protein kinase D inhibitor suppresses AKT on T cells and antagonizes cancer immunotherapy by anti-PD-1. Kazuhide Miyamoto, Hodaka Hayabuchi, Yukiko Tokifuji, Makoto Ando, Nobuyuki Onishi, Tadashi Okamura, Akihiko Yoshimura, Shunsuke Chikuma Int. Immunol., 34, 609-619 (2022). 35849090 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Amp | Ampicillin resistance gene (E. coli) | 3 | Amp | ||||
| Il2 MGI:96548 | interleukin 2 | 3 | Il2<em1(mCherry)Schik> | endonuclease-mediated mutation 1, Shunsuke Chikuma | |||
| Ires | internal ribosomal entry site (EMCV) | 3 | |||||
| mCherry | Red Fluorescent Protein (Discosoma sp.) | 3 | mCherry | ||||
| neo | neomycin resistance gene (E. coli) | 3 | |||||
| SV40 polyA | 3 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | Encephalomyocarditis virus IRES, Discosoma sp. mCherry cDNA, E.coli neo, SV40 polyA, M13 lac promoter CAP binding site, Escherichia coli ampicillin resistance gene, mouse Il2 (NM_008366-3) |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol., 34, 609-619 (2022). |
| Depositor | Shunsuke CHIKUMA (Keio University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |