Strain Data Sheet

RBRC12102

Strain Information

Image
BRC No.RBRC12102
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6.129-H3f3b<em1.1(K36M)Hsas>
Former Common nameH3f3b K36M-flox/K36M-flox
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
A histone H3.3K36M mutation in mice causes an imbalance of histone modifications and defects in chondrocyte differentiation.
Shusaku Abe, Hiroaki Nagatomo, Hiroyuki Sasaki, Takashi Ishiuchi
Epigenetics, 16(10):1123-1134 (2021). 33135541

Histone H3K36me2 and H3K36me3 form a chromatin platform essential for DNMT3A-dependent DNA methylation in mouse oocytes.
Seiichi Yano, Takashi Ishiuchi, Shusaku Abe, Satoshi H Namekawa, Gang Huang, Yoshihiro Ogawa, Hiroyuki Sasaki
Nat. Commun., 13(1):4440 (2022). 35922445

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
H3f3b
MGI:1101768		H3.3 histone B11H3f3b<em1.1(K36M)Hsas> endonuclease-mediated mutation 1.1, Hiroyuki Sasaki
loxP phage P1 loxP11loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNABacteriophage P1 loxP, Mouse A part of H3f3b gene, Streptococcus pyogenes gRNA, Cas9, Saccharomyces cerevisiae Frt
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Epigenetics, 16(10):1123-1134 (2021). Nat. Commun., 13(1):4440 (2022).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorHiroyuki Sasaki (Kyushu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data