Strain Data Sheet
RBRC12122
Strain Information | |
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| Image | |
| BRC No. | RBRC12122 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Iqgap3<tm2.1(cre/ERT2)Itoy> |
| Former Common name | Iqgap3-CreERT2 or Iqgap3-2A-CreERT2 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | 1. The Iqgap3 gene (NCBI Reference Sequence: NM_001033484.1) is located on mouse chromosome 3.Thirty-eight exons have been identified, with the ATG start codon in exon 1 and the TGA stop codon in exon 38.2. In the targeting vector, the TGA stop codon will be replaced with the “2A-CreERT2” cassette.3. To engineer the targeting vector, homology arms will be generated by PCR using BAC clone from the C57BL/6 library as template.4. In the targeting vector, the Neor cassette will be flanked by SDA (self-deletion anchor) sites. DTA will be used for negative selection.5. C57BL/6 ES cells will be used for gene targeting. |
| Strain description | Iqgap3 is a cytosolic scaffold protein mainly expressed in stem/progenitor cells. Iqgap3-CreERT2 mouse strain can induce or deplete genes of interest in Iqgap3+ stem/progenitor cells due to the Cre/LoxP system.Endogenous Iqgap3 expression is intact. The mice do not show abnormality, and the female mice can get pregnant normally. |
| Colony maintenance | 1. Targeted C57BL/6 ES cell clone was injected into C57BL/6 albino embryos, which were then re-implanted into CD-1 pseudo-pregnant females.2. Founder animals were identified by their coat color, their germline transmission was confirmed by breeding with C57BL/6 females and subsequent genotyping of the offspring.3. Heterozygous targeted mice were generated and maintain strain by crossing with wild type C57BL/6 mice. |
| References | Iqgap3-Ras axis drives stem cell proliferation in the stomach corpus during homoeostasis and repair. Junichi Matsuo, Daisuke Douchi, Khine Myint, Naing Naing Mon, Akihiro Yamamura, Kazuyoshi Kohu, Dede Liana Heng, Sabirah Chen, Nur Astiana Mawan, Napat Nuttonmanit, Ying Li, Supriya Srivastava, Shamaine Wei Ting Ho, Nicole Yee Shin Lee, Hong Kai Lee, Makoto Adachi, Atsushi Tamura, Jinmiao Chen, Henry Yang, Ming Teh, Jimmy Bok-Yan So, Wei Peng Yong, Patrick Tan, Khay Guan Yeoh, Linda Shyue Huey Chuang, Sachiko Tsukita, Yoshiaki Ito Gut, 70(10)1833-1846 (2021). 33293280 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| ERT2 | estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A) | 3 | ERT2 | ||||
| Iqgap3 MGI:3028642 | IQ motif containing GTPase activating protein 3 | 3 | Iqgap3<tm2.1(cre/ERT2)Itoy> | targeted mutation 2.1, Yoshiaki Ito | |||
| P2A | porcine teschovirus-1 2A peptide | 3 | P2A | ||||
| cre | Phage P1 Cre recombinase | 3 | cre | ||||
| rox | Bacteriophage D6 rox site | 3 | rox | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | 2A : P2A self-cleaving peptide. This sequence is derived from porcine teschovirus-1.(Tang et al., Vet Res2016.CreERT2 : Fusion protein of Cre-recombinase (Phage P1) and mutated estrogen receptor (human).SDA site (Rox: Phage D6)Tamoxifen induce translocation of CreERT2 into nucleus and remove loxP sequence |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Gut, 70(10)1833-1846 (2021).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. A recipient shall acquire a prior written consent from the Depositor prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. |
| Depositor | Yoshiaki Ito (National University of Singapore) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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