Strain Data Sheet

RBRC12172

Strain Information

Image
BRC No.RBRC12172
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hprt1<tm1Rerf>
Former Common nameHPRT-dup-GFP mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Creation of Mice Bearing a Partial Duplication of HPRT Gene Marked with a GFP Gene and Detection of Revertant Cells In Situ as GFP-Positive Somatic Cells.
Asao Noda, Hirofumi Suemori, Yuko Hirai, Kanya Hamasaki, Yoshiaki Kodama, Hiroshi Mitani, Reid D Landes, Nori Nakamura
PLoS One, 10(8):e0136041 (2015). 26295470

Massive expansion of multiple clones in the mouse hematopoietic system long after whole-body X-irradiation.
Kengo Yoshida, Yasunari Satoh, Arikuni Uchimura, Munechika Misumi, Seishi Kyoizumi, Masataka Taga, Yukiko Matsuda, Asao Noda, Yoichiro Kusunoki
Sci. Rep., 12(1):17276 (2022). 36241679

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)X
Hprt1
MGI:96217		hypoxanthine phosphoribosyltransferase 1XHprt1<tm1Rerf>
MGI:7606970		targeted mutation 1, Radiation Effects Research Foundation
  • hypoxanthine guanine phosphoribosyltransferase partial deficiency(DisGeNET, MedGEN)

  • Lesch-Nyhan syndrome(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)X
    puro puromycin acyltransferase (Streptomyces alboniger)Xpuro

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAマウスゲノム DNA, Streptomyces alboniger Puromycin resistance gene (pPUR Clontech), 大腸菌トランスポゾン Neomycin resistance gene (pSV2 neo AddGene), オワンクラゲ Green Fluorescent Protein gene (pQBI25 和光純薬), ニワトリ、ヒト CMV CAG promoter element（pCAGGS AddGene）
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 10(8):e0136041 (2015). A patent has been granted to the depositor for commercial use of the mouse. Therefore, if the purpose is commercial use, an agreement with the depositor and his affiliated institution is required.
    DepositorAsao Noda (Radiation Effects Research Foundation)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (more than 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. CAGGS MTA (English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data