Strain Data Sheet
RBRC12273
Strain Information | |
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| Image | |
| BRC No. | RBRC12273 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Runx3<tm3.1Itoy> |
| Former Common name | Runx3-R122C, Runx3-R122C/R122C or Runx3R122C/R122C |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | 1. The Runx3 exon2-4 was isolated from C57BL/6 BAC clone using high-fidelity polymerase chain reaction.2. PCR-based Site-direct mutagenesis was performed to introduce the point mutation R122C in exon 3.3. The targeting vector was generated by cloning the genomic fragments containing the intronic region between exon 3 into the polylinker of the target vector NEO-DTA cassette contained a Pgk-Neor (neomycin resistant) gene flanked by LoxP sites and a DTA (diphtheria toxin) cassette for the selection.4. TT2 ES cells will be used for gene targeting.5. The neomycin expression cassette was then excised by crossing the chimera mice with EIIa-Cre mice. (Douchi et al., CMGH 2022)1. Targeted TT2 ES cell clone which has R123C mutation (equivalent of human RUNX3 R122C mutation) in exon 3 of Runx3 gene was injected into C57BL/6 embryos, which were then re-implanted into pseudo-pregnant females.2. Germline transmission was confirmed by genotyping of the offspring.3. Heterozygous targeted mice were generated and maintain strain by crossing with wild type C57BL/6 mice.4. This line was backcrossed to C57BL/6 for more than ten generations by the donating laboratory.5. This line is maintained as homozygote. |
| Strain description | Runx3 is a transcription factor and functions as a developmental regulator. Runx3 is frequently associated with cancer. R122C mutation (equivalent of mouse Runx3 R123C mutation) was originally identified in human gastric cancer. 30 % of Runx3-R122C/R122C homozygote mice show some precancerous metaplasia at 6-month age. The homozygote female mice can get pregnant normally. The homozygote mice can survive more than one year. |
| Colony maintenance | |
| References | A Point Mutation R122C in RUNX3 Promotes the Expansion of Isthmus Stem Cells and Inhibits Their Differentiation in the Stomach. Daisuke Douchi, Akihiro Yamamura, Junichi Matsuo, Jung-Won Lee, Napat Nuttonmanit, Yi Hui Melissa Lim, Kazuto Suda, Mitsuhiro Shimura, Sabirah Chen, ShuChin Pang, Kazuyoshi Kohu, Mari Kaneko, Hiroshi Kiyonari, Atsushi Kaneda, Hideyuki Yoshida, Ichiro Taniuchi, Motomi Osato, Henry Yang, Michiaki Unno, Jimmy Bok-Yan So, Khay Guan Yeoh, Linda Shyue Huey Chuang, Suk-Chul Bae, Yoshiaki Ito Cell. Mol. Gastroenterol. Hepatol., 13(5) 1317-1345 (2022). 35074568 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Runx3 MGI:102672 | runt related transcription factor 3 | 4 | Runx3<tm3.1Itoy> MGI:2179229 | targeted mutation 3.1, Yoshiaki Ito | |||
| loxP | phage P1 loxP | 4 | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Enterobacteria phage P1 LoxP site, mouse Runx3 genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Mol. Gastroenterol. Hepatol., 13(5) 1317-1345 (2022).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. A recipient shall acquire a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. |
| Depositor | Yoshiaki Ito (National University of Singapore) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |