Strain Data Sheet
RBRC12274
Strain Information | |
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| Image | |
| BRC No. | RBRC12274 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Runx3<tm2Itoy> |
| Former Common name | Runx3-R122C-cKI, LSL-Runx3 R122C |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | 1. The Runx3 gene (NCBI Reference Sequence: NM_019732.2) is located on mouse chromosome 4. Six exons have been identified, with the ATG start codon in exon 1 and TAA stop codon in exon 6.2. For the KI model, the “loxP-Endogenous SA of intron 2-Runx3 exon 3~6 CDS-ployA-SDA-Neo-SDA-loxP” cassette was inserted into intron 2, the p.R137C (CGC to TGC) was introduced into exon 3 in 3’ homology arm.3. In the targeting vector, the Neo cassette was flanked by SDA (self-deletion anchor) sites. DTA will be used for negative selection.4. C57BL/6 ES cells will be used for gene targeting.Mouse Runx3 conditional knockin model in C57BL/6N mice.1. Targeted C57BL/6N ES cell clone was injected into C57BL/6 albino embryos, which were then re-implanted into CD-1 pseudo-pregnant females.2. Founder animals were identified by their coat color, their germline transmission was confirmed by breeding with C57BL/6N females and subsequent genotyping of the offspring.3. Heterozygous targeted mice were generated, and strains maintained by crossing with wild type C57BL/6N mice. |
| Strain description | Runx3 is a transcription factor and functions as a developmental regulator. Runx3 is frequently associated with cancer. R122C mutation was identified in human gastric cancer. LSL-Runx3 R122C mouse strain expresses wild-type Runx3, which is regulated by endogenous Runx3 promoter activity, and the strain does not show any phenotype. By crossing with promoter-specific Cre mouse, wild-type Runx3 expression will be replaced with the mouse equivalent of R122C in Cre-expressing cells (i.e. R123C in mouse, henceforth referred as R122C for clarity and historical reasons). The homozygote female mice can get pregnant normally. The homozygote mice can survive more than one year. |
| Colony maintenance | |
| References | A Point Mutation R122C in RUNX3 Promotes the Expansion of Isthmus Stem Cells and Inhibits Their Differentiation in the Stomach. Daisuke Douchi, Akihiro Yamamura, Junichi Matsuo, Jung-Won Lee, Napat Nuttonmanit, Yi Hui Melissa Lim, Kazuto Suda, Mitsuhiro Shimura, Sabirah Chen, ShuChin Pang, Kazuyoshi Kohu, Mari Kaneko, Hiroshi Kiyonari, Atsushi Kaneda, Hideyuki Yoshida, Ichiro Taniuchi, Motomi Osato, Henry Yang, Michiaki Unno, Jimmy Bok-Yan So, Khay Guan Yeoh, Linda Shyue Huey Chuang, Suk-Chul Bae, Yoshiaki Ito Cell. Mol. Gastroenterol. Hepatol., 13(5) 1317-1345 (2022). 35074568 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Runx3 MGI:102672 | runt related transcription factor 3 | 4 | Runx3<tm2Itoy> | targeted mutation 2, Yoshiaki Ito | |||
| loxP | phage P1 loxP | 4 | loxP | ||||
| rox | Bacteriophage D6 rox site | 4 | rox | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | loxP Enterobacteria phage P1 由来, ウシ BGH poly A, Rox Bacterial virus(Enterobacteria phage D6) |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Mol. Gastroenterol. Hepatol., 13(5) 1317-1345 (2022).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. A recipient shall acquire a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. |
| Depositor | Yoshiaki Ito (National University of Singapore) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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