Strain Data Sheet

RBRC12279

Strain Information

Image
BRC No.RBRC12279
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Rock1<tm1.1Ttti>
Former Common nameROCK1flox/flox
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Conditional deficiency of Rho-associated kinases disrupts endothelial cell junctions and impairs respiratory function in adult mice.
Takahiro Akamine, Takeshi Terabayashi, Takako Sasaki, Riku Hayashi, Ichitaro Abe, Fumihiro Hirayama, Shin-Ichi Nureki, Masahito Ikawa, Haruhiko Miyata, Akinori Tokunaga, Takashi Kobayashi, Katsuhiro Hanada, Dean Thumkeo, Shuh Narumiya, Toshimasa Ishizaki
FEBS Open Bio., 14(6):906-921 (2024). 38604990

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site18Frt
Rock1
MGI:107927		Rho-associated coiled-coil containing protein kinase 118Rock1<tm1.1Ttti> targeted mutation 1.1, Toshimasa Ishizaki
loxP phage P1 loxP18loxP
loxP phage P1 loxP18loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Rock1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Open Bio., doi:10.1002/2211-5463.13802.For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorToshimasa Ishizaki (Oita University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data