Strain Data Sheet

RBRC12283

Strain Information

Image
BRC No.RBRC12283
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Rag2<em2(tTA)Rygo>
Former Common nameRAG2-tTA
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
B Cells of Early-life Origin Defined by RAG2-based Lymphoid Cell Tracking under Native Hematopoietic Conditions.
Keiko Fujisaki, Shogo Okazaki, Shuhei Ogawa, Miyama Takeda, Eiji Sugihara, Kenichi Imai, Seiya Mizuno, Satoru Takahashi, Ryo Goitsuka
J. Immunol., 213(3):296-305 (2024). 38874543

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rag2
MGI:97849		recombination activating gene 22Rag2<em2(rtTA)Rygo> endonuclease mediated mutation 2, Ryo Goitsuka
  • combined immunodeficiency with skin granulomas(MedGEN)

  • Omenn syndrome(DisGeNET, MedGEN)

  • severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, NK cell-positive(DisGeNET, MedGEN)
    • tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)2tTA

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAE.coli TetR, Herpes Simplex virus VP16, Simian Virus 40 SV40 poly A, mouse Rag2 genomic DNA
    Research applicationTet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 213(3):296-305 (2024).
    DepositorRyo Goitsuka (Tokyo University of Science)
    Strain Statusan icon for Live miceLive mice
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data