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G-CaMP-HS

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP-HS.

Catalog number RDB14607
Resource name G-CaMP-HS
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. GCaMP-hyper sensitive (G-CaMP-HS) protein became more stable and stronger fluorescence than GCaMP2 in transfected mammal cells, and a GCaMP-HS is better suited to detect Ca2+ transients in vivo imaging of neuronal activities in zebrafish.
Comment Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AKE44628.1 (DDBJ accession number)
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
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External Database
Rat Calm2 Chickin MLCK Aequorea victoria GFP

        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Muto, A. et al. Proc. Nati. Acad. Sci. USA 108 (13): 5425-5430, 2011) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
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        提供条件 研究成果の公表にあたって寄託者の指定する文献(Muto, A. et al. Proc. Nati. Acad. Sci. USA 108 (13): 5425-5430, 2011)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用(営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等)の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。
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        手続きの概要は、「提供申込みについて[link]」をご覧ください。

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        RDB14607 G-CaMP-HS DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The G-CaMP-HS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14607).

        Reference section:

        Muto, A., Ohkura, M., Kotani, T., Higashijima, S., Nakai, J., Kawakami, K., Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). PMID 21383146. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


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        Featured content

        Featured content G-CaMP Calcium Sensor Clones (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14607_A7Bgp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward (Pr0016)
        Region: CMV pro,insert 5'(G-CaMP-HS 5')
        Sequence file: RDB14607_A7Bga.seq check
        >D04021A2_A7Bg_1_CMV-Forward_B10_04_ABI24.ab1
            1 NNNNNNNNNN NNCCNNTTGN NGCNNATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
           61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGCC
          121 ACCATGCGGG GTTCTCATCA TCATCATCAT CATGGTATGG CTAGCATGAC TGGTGGACAG
          181 CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC TCGCCACCAT GGTCGACTCA
          241 TCACGTCGTA AGTGGAATAA GACAGGTCAC GCAGTCAGAG CTATAGGTCG GCTGAGCTCA
          301 CTCGAGAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGC GAACTTCAAG
          361 ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCT ACCACTACCA GCAGAACACC
          421 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGTA
          481 CTTTCGAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC
          541 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGCG GTACCGGAGG GAGCATGGTG
          601 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
          661 GTAAACGGCC ACAAGTTCAG CGTGCGCGGC GAGGGTGANG GCGATGCCAC CNACGGCAAG
          721 CTGANCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGNCC CACCCTCGTG
          781 ACCACCCTGA CCTACGGCGT GCAGNGCTTC AGCCGCTACC CCGANCACAT GAAGCAGCAC
          841 GACTTCTTCN AGTCCNCCNT GNCCGANNGN TACNTCNAGG ANCGCANCNT CTNCNNCNNG
          901 GANGANGGNN CCTACNANAN CCNCGNNNAN GNNNNNTNNN NGGGCNANNN CCNNNNNNAN
        961 NNNNTCNNNC TNNNNNNNNT CGNCTNNNNN NNNNN
        //
        Primer: SV40polyA-R (Pr0109)
        Region: insert 3'(G-CaMP-HS 3')
        Sequence file: RDB14607_A7Bgb.seq check
        >D04021A2_A7Bg_1_SV40polyA-R_B11_05_ABI24.ab1
            1 NNNNNNNNNN NNNNTNNNNN ANNNNNNCTC TACNAATGTG GTATGGCTGA TTATGATCTA
           61 GAGTCGCGGC CGCTCACTTC GCTGTCATCA TTTGTACAAA CTCTTCGTAG TTTACCTGAC
          121 CATCCCCATC GATGTCTGCT TCCCTGATCA TTTCATCAAC CTCTTCATCT GTTAACTTCT
          181 CTCCAAGGTT TGTCATCACG TGGCGAAGCT CTGCTGCACT GATGTAGCCA TTGCCATCCT
          241 TATCAAACAC ACGGAACGCT TCTCTAATTT CTTCTTCACT GTCTGTGTCT TTCATTTTTC
          301 TTGCCATCAT TGTCAGGAAC TCAGGGAAGT CGATTGTGCC ATTACCGTCG GCATCTACTT
          361 CATTGATCAT GTCCTGCAGC TCTGCTTCTG TGGGGTTCTG CCCCAGAGAC CGCATCACCG
          421 TCCCCAGCTC CTTGGTTGTT ATTGTCCCAT CCCCGTCCTT GTCAAATAGG GAGAAAGCCT
          481 CTTTAAATTC TGCGATCTGC TCTTCAGTCA GTTGGTCACG CGTGTTGTAC TCCAGCTTGT
          541 GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA
          601 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GGTGCCGTCG TCCTTGAAGA
          661 AGANGGTGCG CTCCTGGATG TAGCCTTCGG GCATGGCGGA NTTGAAGAAG TCGTGCTGCT
          721 TCATGTGGTC GGGGTAGCGG CTGAAGCACT GCACGCCGTA NGTCANGGTG GTCACGAGGG
          781 TGGGCCANGG CACGGGCAGC TTGCCGGNGG TGCAGANGAA CTTCNNGGTC AGCTNGNCGT
          841 TGGTGGNATC GCCCTCACCC TCGCCGCGCA CGCTGAACTT GTGGNCGNTT ACNTCNCCNT
          901 CCANCTCGNN CNNNNNGGGC ACNNNCCNNG NGAANAGCTN CNCGCCCTTG NNNNNCNNGN
        961 NNCNNNNNNN ANNNNCCNTN NNANNGNNCN NCNATNNNNA NNNNGNTNNN NNNNNNN
        //
        Primer: ECFP-C_F1 (Pr0925)
        Region: insert mid(G-CaMP-HS )
        Sequence file: RDB14607_A7Bgc.seq check
        >14607_14607_B3GbF_ECFP-C_F1_E01_05_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NCGNNCNCNT NNNNNTGCTG GANTTCGTGN CCGCCGCCGG
           61 GNTCACTCTC GGCATGGNCG AGCTGTACAA GGGCGGTACC GGAGGGAGCA TGGTGAGCAA
          121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
          181 CGGCCACAAG TTCAGCGTGC GCGGCGAGGG TGAGGGCGAT GCCACCAACG GCAAGCTGAC
          241 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
          301 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
          361 CTTCAAGTCC GCCATGCCCG AAGGCTACAT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
        421 CGGNNNNNNN NNN
        //
        Primer: SV40pro_F_V2 (Pr0154)
        Region: NeoR_KanR
        Sequence file: RDB14607_A7Bgd.seq check
        >D04021A2_A7Bg_1_SV40pro_F_V2_B12_06_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNANNCT TTTGNNNAGN NCGANCNNNA GNCAGGATGA
           61 GGANCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG
          121 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG
          181 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC
          241 CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT
          301 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA
          361 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG
          421 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA
          481 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT
          541 GATCTGNANG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG
          601 AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC
          661 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC
          721 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AANAGCTTGG CGGCGAATGG
          781 NNTGANCGCT TCCTCNNGCT TTTACNGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT
          841 TCTATCNNCT TCNNGACGAN TTCTNCNGAN CGGGNANTCT GGGGTTCNAA ATGACNNACC
          901 NAGCGACGCC NNACCTGNCA TCNCGAGATT TCNANNNCNN CGNCGNCNTN CTATGAANNN
          961 NNNNNCTTCN GNANNNGTTT NNCNGGNNNN NNNGNNNGGA TGANNNNNCN NNNNNGGGGN
        1021 NNNNCNNGNN NGNNNNNNNN CNNCCCNNCC NNNNGGGGGN NNNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Muto, A., Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). PMID 21383146. [link to RRC of NBRP]

        2024.03.26

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