RDB15453 - tFucci(CA)2.1/pCSII-CMV

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内)

[open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. 付加的提供条件：レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

How to cite this biological resource

Tips

QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15453	tFucci(CA)2.1/pCSII-CMV	DNA solution

Featured content	Lentiviral plasmid list (English text)
Featured content	Fucci-related materials (en) (English text)
Featured content	Fucci-related materials (ja) (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: mCherry-C_R (Pr0517) Region: insert 5' (mCherry), CMV pro_2 Sequence file: RDB15453_A7Jvg.seq
>D05018B4_2_A7Jv_mCherry-C_R_D01_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN GTTGNNGGCG CCGGGCANCT GCACGGGCTT 61 CTTGGCCTTG TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG 121 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC 181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG 241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC 301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG 361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACATGAA 421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT 481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG 541 GCCGTTCACG GAGCCCTCCA TGTGCACCTT GAAGCGCATG AACTCCTTGA TGATGGCCAT 601 GTTATCCTCC TCGCCCTTGC TCACCATGGT GGATCCTATG CTAGCGGATC TGACGGTTCA 661 CTAAACCAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA 721 ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CNAAACAAAC 781 TCCCATTGAC GTCNATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC 841 GCNCATTGAT GTACTGCCNA AACCGCATCA CCATGGNAAT AGCGATGANT AANACGTANA 901 TGTACTGCNN AGNNNNAAAG TNCNNTAANG NCATGTNCNG GNNNTNANGN CNNGGNGGNN 961 NNTTTANNNN CATTGNNNNN NNNNN //
Primer: PRE_rev (Pr0250) Region: PRE, insert 3' (hGem(1_110), AmCyan1) Sequence file: RDB15453_A7Jvh.seq
>D05018B4_2_A7Jv_PRE_rev_E02_14_ABI24.ab1 1 NNNNNNNNNN NGCGNNNNGG AGCANCATAG TTAAGANTAC CAGTCAATCT TTCNCAAATT 61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTACA 121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA 181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG 241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT 301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT 361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT 421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCGA 481 AGGGCACCAC GGAGGTGATG TGGGCCACGG CGTGCTCGGT CAGCTGCACG CTGTTGCCGC 541 CCTTGTCCAG GTCGGTTCTG GCGATGCGGT GCTCCACCAC GTGGTTGGGG GGCATGGTCA 601 CGGGCTTCTT GGTCTTGTAG GAGGTGTGGA ACTGGCATCT GTAGTTGCCG CCGCCCTGCA 661 GCATCAGGAA GGCGGTCACG TCGCCCTTCA AGATGCCGTC GCACACGGTC ATCTTCTCGA 721 ANGANGGGTC CCAGCCGGTG GTCTTCTTGG NCATCACGGG GCCGTCGGCG GGGAANTTCA 781 CGCCGNGGAA NGTGGANTTG TGCTCNAAGC AGTTGCCCTT CNNNTGATCT CCCNGCTGGC 841 GGTGGNCNCG CCGCCNNCCN CNNNNNGAAN GNTNNCTCGN NNNNNTGNCN NCGGGGANNN 901 NCNGCTTNAA NNANNNNGNN NTGCTNNNGG GGNNNNNNNN NAANNANNNG NTNNNNNNAC 961 NTGNNNNNNN NNN //
Primer: mRFP1-inner1 (Pr0443) Region: insert mid (mCherry, hCdt1(1_100)Cy(-)) Sequence file: RDB15453_A7Jvi.seq
>15453_15453_B1K5F_2_mRFP1-inner1_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNCNNN GNNTCCTCCC TGCAGGANGG CGAGTTCATC TACAAGGTGA 61 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT 121 GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC 181 AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG 241 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT 301 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA 361 CCGGCGGCAT GGACGAGCTG TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGATGG 421 AGCAGCGCCG CGTCACCGAC TTCTTCGCGC GCCGCCGCCC CGGGCCCCCC CGCATCGCGC 481 CGCCCAAGCT GGCCTGCCGC ACCCCCAGCC CCGCCAGGCC CGCACTCCGC GCCCCGGCCT 541 CCGCTACCAG TGGCAGCCGC AAGCGCGCCC GCCCGCCCGC CGCCCCCGGA NGCGNNCAGG 601 CCAGGNCACC GGCCCGCGCG GCCGCGCGGC TGTCGGNGGA NNANGNTTCC AGCCCCAGTA 661 CCCCCNANGC CCNNNANNTC CNNNNNNGNC CNNCTNNNGN NNNNNNNNN //
Primer: mCherry-C_F (Pr0453) Region: insert mid (mCherry, hCdt1(1_100)Cy(-), P2A, AmCyan1) Sequence file: RDB15453_A7Jvj.seq
>D05018B4_2_A7Jv_mCherry-C_F_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGCT GCCNGGCGCC TACNACGTCA ACATCAAGTT GGACATCACC 61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC 121 ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC GCTCGAGATG 181 GAGCAGCGCC GCGTCACCGA CTTCTTCGCG CGCCGCCGCC CCGGGCCCCC CCGCATCGCG 241 CCGCCCAAGC TGGCCTGCCG CACCCCCAGC CCCGCCAGGC CCGCACTCCG CGCCCCGGCC 301 TCCGCTACCA GTGGCAGCCG CAAGCGCGCC CGCCCGCCCG CCGCCCCCGG ACGCGACCAG 361 GCCAGGCCAC CGGCCCGCGC GGCCGCGCGG CTGTCGGTGG ACGAGGTTTC CAGCCCCAGT 421 ACCCCCGAGG CCCCAGACAT CCCAGCCTGC CCTTCTCCGG GCCAGAAGAT AAAGAAAACC 481 GGTCGCGCCA AGCGCGGCAG CGGCGCCACC AACTTCAGCC TGCTGAAGCA GGCCGGCGAC 541 GTGGAGGAGA ACCCCGGCCC CCGCGAATTC GCCACCATGG CCCTGTCCAA CAAGTTCATC 601 GGCGACGACA TGAAGATGAC CTACCACATG GACGGCTGCG TGAACGGCCA CTACTTCACC 661 GTGAAGGGCG AGGGCAGCGG CAAGCCCTAC GAGGGCACCC AGACCTCCAC CTTCAAGGTG 721 ACCATGGCCA ACGGCGGCCC CCTGGCCTTC TCCTTCGACA TCCTGTCCAC CGTGTTCATG 781 TACGGCAACC GCTGCTTCAC CGCCNACNNC ACCAGCATGC CCGANTACTT CNAGCANGCC 841 TTCCCCGANG GCATGTCCTA CGAGAGAANC TTCACCTACG ANGNNGGNGG CGNGGCCNCC 901 GNCAGCNGGG ANNNCAGCCN GAANGGNAAC TGCTNCNAGC ACAAGTNCNN NTTNCNCGGN 961 GNNNNNNNNC CNNNNNANNG NCCNNNNNNN GNNNNAGANN ANNNNNNNNN N //
Primer: AmCyan1-H_F (Pr1071) Region: insert mid (AmCyan1, hCdt1(1_110)) Sequence file: RDB15453_A7Jvk.seq
>15453_15453_B1K5F_2_AmCyan1-H_F_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGG CNNCCNGNNC TCCNCCTTCA AGGTGACCAT GGCCAACGGC 61 GGCCCCCTGG CCTTCTCCTT CGACATCCTG TCCACCGTGT TCATGTACGG CAACCGCTGC 121 TTCACCGCCT ACCCCACCAG CATGCCCGAC TACTTCAAGC AGGCCTTCCC CGACGGCATG 181 TCCTACGAGA GAACCTTCAC CTACGAGGAC GGCGGCGTGG CCACCGCCAG CTGGGAGATC 241 AGCCTGAAGG GCAACTGCTT CGAGCACAAG TCCACCTTCC ACGGCGTGAA CTTCCCCGCC 301 GACGGCCCCG TGATGGCCAA GAAGACCACC GGCTGGGACC CCTCCTTCGA GAAGATGACC 361 GTGTGCGACG GCATCTTGAA GGGCGACGTG ACCGCCTTCC TGATGCTGCA GGGCGGCGGC 421 AACTACAGAT GCCAGTTCCA CACCTCCTAC AAGACCAAGA AGCCCGTGAC CATGCCCCCC 481 AACCACGTGG TGGAGCACCG CATCGCCAGA ACCGACCTGG ACAAGGGCGG CAACAGCGTG 541 CAGCTGACCG AGCACGCCGT GGCCCACATC ACCTCCGTGG TGCCCTTCGG ATATCCATCA 601 CACTGGCGGC CGCTCGAGAT GANNCCCNGT ATGAAGCANN NNNAGAAGAA ATCAAAGAGA 661 ATATAAAGAA TAGTTNTGTC CCNNGAANAN NTCTGNNNNN NNNTCNNNNN NNGCNTCTNN 721 NNNNNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi, 5'LTR, CMV pro_1, CMV en_1 Sequence file: RDB15453_A7Jvl.seq
>15453_15453_B1K5F_2_HIV-B_R_E02_14_ABI24.ab1 1 NNNNNNNNNC NNNNNANNNT TTTGGCGTAC TCNCCAGTCG CCGCCCCTCG CCTCTTGCCG 61 TGCGCGCTTC AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT 121 TCAAGTCCCT GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG 181 GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG 241 CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA 301 GATCTGGTCT AACCAGAGAG ACCCAGTACA GGCAAAACGC GCTGCTTATA TAGACCTCCC 361 ACCGTACACG CCTACCGCCC ATTTGCGTCA ATGGGGCGGA GTTGTTACGA CATTTTGGAA 421 AGTCCCGTTG ATTTTGGTGC CAAAACAAAC TCCCATTGAC GTCAATGGGG TGGAGACTTG 481 GAAATCCCCG TGAGTCAAAC CGCTATCCAC GCCCATTGAT GTACTGCCAA AACCGCATCA 541 CCATGGTAAT AGCGATGACT AATACGTAGA TGTACTGCCA AGTANGAAAG TCCCATAAGG 601 TCATGTACTG GGCATAATGC CAGGCGGGCC ATTTACCGTC NTTGACGTCN ATANGGGGCG 661 TACTTGGNAT ANGANANACT TGANGTACTG NCNNNNGGNN NNNTNNNNNN NNNN //
Primer: BGH_rev2 (Pr0606) Region: 3'LTR, PRE Sequence file: RDB15453_A7Jvm.seq
>D05018B4_2_A7Jv_BGH_rev2_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGGNNGNN GGGCNNACAA CAGATGGCTG GCAACTAGAA 61 GGCACAGTCG AGGCTGNTCN NCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA 121 CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC 181 TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA 241 GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC 301 TCGGGCCATG TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC 361 CGGGTCATCC ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT 421 GGCTTCTTCT ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG 481 TGGATATCTG ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT 541 GTGGTAGATC CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG 601 TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT 661 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA 721 GGCACAATCA GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG 781 GTCTCGACGG TATCGATGCG GGGANGCGGC CCAAAGGGAN ATCCGACTCG TCTGANGGCG 841 AANGCGAANA NGCGGAAGAN GCCGCANANC CNGCAGCANG CCNNNGGAAN GNANGGTCCG 901 CNNGATTGAG GNNCNAANGG ACGTANCANA ANGGACGNNC CNCGCANAAN CCANGNNGNN 961 NCACAGGNNA NCAGCCNTNN NAANGNNNNN NNCNTNNNCN NNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: Zeo, SV40 pA Sequence file: RDB15453_A7Jvn.seq
>D05018B4_2_A7Jv_SV40pro_F_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NANNNNTGAN GANGCTTTTT TGGANGNCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCNTTT TCGGATCTGA TCAGCACGTG TTGACAATTA 121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC 181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 781 CNAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCN ANCTCTAGCT AGAGCTTGGC 841 GTAATCATGG TCNTAGCTGT TTCCTGTGTG AAATTGNTAT CCGCTCACAA TTCCNCACNA 901 CATACGAGCC NGAANCATAA AGNGNAAAGN CNGGGGNGNC NAANGANTGA GCNNACTCNC 961 ATTNATTGCN NNGCGCTCAC NNCCNNNTTT NCANNNNGNN AANNCTNNNN NGNNNNNNGC 1021 NTNNNNGNNN NNNNCNNNNN NNNNGGNNNN NNNNNN //

tFucci(CA)2.1/pCSII-CMV

Distribution information

How to cite this biological resource

Tips

QC test results

References