Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator
Fucci5
Ando, R., et al. Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513.
DNA materials
| Cat. no. | Resource name | Description |
|---|---|---|
| RDB18220 | tFucci(SA)5/pCS2 | CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3 |
| RDB18221 | tFucci(CA)5/pCS2 | CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3 |
Cell line
| Cat. no. | Resource name |
|---|---|
| RCB4917 | HeLa/Fucci(SA)5#16 |
| RCB4919 | HeLa/Fucci(CA)5#16 |
Cell cycle indicator Fucci(CA) and Fucci(SA)
Sakaue-Sawano, A. et al., Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.
DNA materials
| Cat. no. | Resource name | Description |
|---|---|---|
| RDB15459 | mCherry-hCdt1(1/100)Cy(-)/pcDNA3 | CMV promoter, hCdt1, mCherry |
| RDB15273 | mCherry-hCdt1(30/120) / pCSII-EF | EF-1α promoter, hCtd1, mCherry, lentivirus vector |
| RDB15441 | mCherry-hCdt1(1/100)Cy(-)/pCSII-EF | EF-1α promoter, hCtd1, mCherry, lentivirus vector |
| RDB15442 | mCherry-hCdt1(1/100)/pCSII-EF | EF-1α promoter, hCtd1, mCherry, lentivirus vector |
| RDB15271 | mVenus-hGeminin(1/110) / pCSII-EF | EF-1α promoter, hGeminin, mVenus, lentivirus vector |
| RDB15446 | tFucci(CA)2/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector |
| RDB15452 | tFucci(CA)2/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector |
| RDB15447 | tFucci(CA)2.1/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector |
| RDB15453 | tFucci(CA)2.1/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector |
| RDB15448 | tFucci(CA)2.2/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector |
| RDB15454 | tFucci(CA)2.2/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector |
| RDB15443 | tFucci(SA)2/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector |
| RDB15449 | tFucci(SA)2/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector |
| RDB15444 | tFucci(SA)2.1/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector |
| RDB15450 | tFucci(SA)2.1/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector |
| RDB15445 | tFucci(SA)2.2/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector |
| RDB15451 | tFucci(SA)2.2/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector |
Cell line
| Cat. no. | Resource name |
|---|---|
| RCB4676 | HeLa/Fucci(CA)2 |
| RCB4675 | NMuMG/Fucci(CA)2.1 |
Cell cycle indicator Fucci2a
Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018). PMID 30458140.
Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.
DNA materials
| Cat. no. | Resource name | Description |
|---|---|---|
| RDB16057 | pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a | CAG promoter, Arl13b, Cerulean, hGem(1/110), mVenus, hCdt1(30/120), mCherry |
| RDB16058 | pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a | >CAG promoter, Arl13b, Cerulean, hGem(1/110), mVenus, hCdt1(30/120), mCherry |
| RDB16059 | pCAG-Arl13bCerulean-Fucci2a | >CAG promoter, Arl13b, Cerulean, hGem(1/110), mVenus, hCdt1(30/120), mCherry |
| RDB13080 | pCAG-Fucci2a | CAG promoter, hGem(1/110), mVenus, hCdt1(30/120), mCherry |
| RDB13081 | pROSA-floxNeo-Fucci2a | CAG promoter, hGem(1/110), mVenus, hCdt1(30/120), mCherry |
| RDB16056 | pCDNA5-Frt-Fucci2a | CMV promoter, hGem(1/110), mVenus, hCdt1(30/120), mCherry |
Mouse
| Cat. no. | Resource name |
|---|---|
| RBRC 06511 | B6;129-Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn> |
Cell line
| Cat. no. | Resource name |
|---|---|
| AES0223 | MESC Fucci2aR |
| AES0224 | MESC Fucci2a |
| RCB5029 | NIH3T3 Flp Arl13bCerulean-Fucci2a |
| RCB5030 | NIH3T3 Flp Fucci2a |
| AES0225 | MESC Arl13b-Fucci2aR |
| AES0226 | MESC Arl13b-Fucci2a |
Cell cycle indicator Fucci2
Sakaue-Sawano, A. et al., BMC Cell Biol. 12: 2 (2011). PMID 21226962.
Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008). PMID 9101468.
DNA materials
| Cat. no. | Resource name | Description |
|---|---|---|
| RDB15457 | mCherry-hCdt1(30/120)/pcDNA3 | CMV promoter, hCdt1, mCherry |
| RDB15458 | mVenus-hGeminin(1/110)/pcDNA3 | CMV promoter, hGeminin, mVenus |
| RDB15273 | mCherry-hCdt1(30/120) / pCSII-EF | EF-1α promoter, hCtd1, mCherry, lentivirus vector |
| RDB15271 | mVenus-hGeminin(1/110) / pCSII-EF | EF-1α promoter, hGeminin, mVenus, lentivirus vector |
| RDB15274 | AmCyan-hGem(1/110) / pCSII-EF | EF-1α promoter, hGeminin, AmCyan, lentivirus vector |
Cell line
| Cat. no. | Resource name |
|---|---|
| RCB2867 | HeLa/Fucci2 |
| RCB2868 | NMuMG/Fucci2 |
Cell cycle indicator Fucci
Sakaue-Sawano, A. et al., Development 140 (22): 4624-4632 (2013). PMID 24154524.
Aiba, Y, et al., Proc. Natl. Acad. Sci. USA. 107 (27): 12192-12197 (2010). PMID 20547847.
Sakaue-Sawano, A., Cell 132 (3): 487-498 (2008). PMID 18267078.
DNA materials
| Cat. no. | Resource name | Description |
|---|---|---|
| RDB15267 | mKO2-hCdt1(30/120) / pCSII-EF | EF-1α promoter, hCtd1, mKO2, lentivirus vector |
| RDB15268 | mAG-hGeminin(1/110) / pCSII-EF | EF-1α promoter, hGeminin, mAG, lentivirus vector |
Mouse
| Cat. no. | Resource name |
|---|---|
| RBRC02706 | B6.Cg-Tg (FucciS/G2/M) #504Bsi |
| RBRC02707 | B6.Cg-Tg (FucciG1) #596Bsi |
| RBRC02704 | B6.Cg-Tg(FucciS/G2/M)#474Bsi |
| RBRC02708 | B6.Cg-Tg(FucciG1)#610Bsi |
| RBRC02705 | B6.Cg-Tg(FucciS/G2/M)#492Bsi |
| RBRC02709 | B6.Cg-Tg(FucciG1)#639Bsi |
Cell line
| Cat. no. | Resource name |
|---|---|
| RCB2812 | HeLa/Fucci |
| RCB2813 | NMuMG/Fucci |
| RCB2814 | COS/Fucci |
Cell cycle indicator
Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.
DNA materials
| Cat. no. | Resource name | Description |
|---|---|---|
| RDB15269 | mCherry-hGeminin(1/110) / pCSII-EF | EF-1α promoter, hGeminin, mCherry, lentivirus vector |
| RDB15270 | mCherry-hGeminin(1/60) / pCSII-EF | EF-1α promoter, hGeminin, mCherry, lentivirus vector |
| RDB15272 | mVenus-hGeminin(1/60) / pCSII-EF | EF-1α promoter, hGeminin, mVenus, lentivirus vector |
| RDB15274 | AmCyan-hGem(1/110)/pCSII-EF | EF-1α promoter, hGeminin, AmCyan, lentivirus vector |
Tol2 plasmid
Sugiyama, M. et al., Proc. Natl. Acad. Sci. USA. 106 (49): 20812-20817 (2009). PMID 19923430.
DNA materials
| Cat. no. | Resource name |
|---|---|
| RDB15278 | mCherry-zCdt1(1/190) / pT2KXIGdeltain |
| RDB15279 | mVenus-zGeminin (1/100) / pT2KXIGdeltain |
| RDB15275 | mKO2-zCdt1(1/190) / pT2KXIGdeltain |
| RDB15276 | mAG-zGeminin(1/100) / pT2KXIGdeltain |
| RDB15277 | mAG-hGeminin (1/60) / pT2KXIGdeltain |
Other Fucci related materials
RIKEN Center for Brain Science (CBS), Dr. Miyawaki lab.
References
Important notice regarding nomenclature of Fucci
- Please read this statement by Dr. Miyawaki [link]
Mouse of the Month
(GRP0044e 2017.09.18 T.M.)
2024.09.13
