RDB18987 - pEF-Tim3-Fc

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pEF-Tim3-Fc (#RDB18987)

Mammalian expression vector of the extracellular region of mouse Havcr2 (Tim3) (aa 1-174).

Clone info.	Mammalian expression vector of the extracellular region of mouse hepatitis A virus cellular receptor 2 (Havcr2, Aliases: Tim3) (aa 1-174) with a splice donor (GTAAGAA). See Fig.3f of Miyanishi, M. et al. Nature, 450 (7168): 435-439, 2007.
Comment	Expression was confirmed by the depositor with Western blotting.
Vector backbone	pEF-Fc (RDB18974) (plasmid)
Selectable markers	Ampicillin
Gene/insert name	mouse Havcr2 cDNA
Depositor\|Developer	Nagata, Shigekazu \|

Sequence (full)	RDB18987zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18987	pEF-Tim3-Fc	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-Tim3-Fc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18987).

Reference section:

Miyanishi, M., Tada, K., Koike, M., Uchiyama, Y., Kitamura, T., Nagata, S., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18987_B1Drp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro, insert(mTim3(aa1-174),splice donor site),IgG Fc-region Sequence file: RDB18987_B1Dra.seq
>D07990C6_B1Dr_1_EF1a-C_F_A05_02_ABI24.ab1 1 NNNNNNNNNN NNNTNNNCCN TTTTTNANTT TGNNNCTTGG TTNNTTCTCN AGCCTCAGAC 61 AGTGGTTCAA AGTTTTTTTC TTCCNTTTCA GGTGTCGTGA GGAATTCTCT AGAGATCCCT 121 CGACCTCGAG ATCCATCGGG CCCCCCCTCG AGGTCGACCA AGTACTCATG TTTTCAGGTC 181 TTACCCTCAA CTGTGTCCTG CTGCTGCTGC AACTACTACT TGCAAGGTCA TTGGAAAATG 241 CTTATGTGTT TGAGGTTGGT AAGAATGCCT ATCTGCCCTG CAGTTACACT CTATCTACAC 301 CTGGGGCACT TGTGCCTATG TGCTGGGGCA AGGGATTCTG TCCTTGGTCA CAGTGTACCA 361 ACGAGTTGCT CAGAACTGAT GAAAGAAATG TGACATATCA GAAATCCAGC AGATACCAGC 421 TAAAGGGCGA TCTCAACAAA GGAGACGTGT CTCTGATCAT AAAGAATGTG ACTCTGGATG 481 ACCATGGGAC CTACTGCTGC AGGATACAGT TCCCTGGTCT TATGAATGAT AAAAAATTAG 541 AACTGAAATT AGACATCAAA GCAGCCAAGG TCACTCCAGC TCAGACTGCC CATGGGGACT 601 CTACTACAGC TTCTCCAAGA ACCCTAACCA CGGAGAGAAA TGGTTCAGAG ACACAGACAC 661 TGGTGACCCT CCATAATAAC AATGGAACAA GTAAGAAGAT ATCGAATTCC TGCAGCCCGG 721 GGGATCCACT AGTTCTATCC TGCCTGGACG CATCCCGGCT ATGCAGTCCC AGTCCAGGGC 781 AGCAAGGCAG GCCCCGTCTG CCTCTTCACC CGGANGCCTC TGCCCGCCCC ACTCATGCTC 841 ANGNANANGG TCTTCTGGCT TTTTNCCNAN GCTCTGGGCA NGCACAGGNT ANGNGCCCCT 901 AACCCANGCC CNGCACACAA AGGGGCANGN GCTGGGCTCA NANCTGNCNA GANNCNTATC 961 CNGNNNGNNC CTGCCCCTGA CCTAANCCCN CCCCAAANNN NNAACNNTCC ANNCCNNCNN 1021 NNNNNNNN //
Primer: hIgG-genome_R1 (Pr1105) Region: IgG Fc-region,insert 3'(splice donor site,mTim3(aa1-174) 3') Sequence file: RDB18987_B1Drb.seq
>D07990C6_B1Dr_1_hlgG-genome_R1_C05_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NTNTTGNNNG GTCTGANCCN NGCACCTGCC CCTTTGTGTG 61 CAGGGCCTGG GTTAGGGGCA CCTAGCCTGT GCCTGCCCAG AGCCTGGGGA AAAAGCCAGA 121 AGACCCTCTC CCTGAGCATG AGTGGGGCGG GCAGAGGCCT CCGGGTGAAG AGGCAGACGG 181 GGCCTGCCTT GCTGCCCTGG ACTGGGACTG CATAGCCGGG ATGCGTCCAG GCAGGATAGA 241 ACTAGTGGAT CCCCCGGGCT GCAGGAATTC GATATCTTCT TACTTGTTCC ATTGTTATTA 301 TGGAGGGTCA CCAGTGTCTG TGTCTCTGAA CCATTTCTCT CCGTGGTTAG GGTTCTTGGA 361 GAAGCTGTAG TAGAGTCCCC ATGGGCAGTC TGAGCTGGAG TGACCTTGGC TGCTTTGATG 421 TCTAATTTCA GTTCTAATTT TTTATCATTC ATAAGACCAG GGAACTGTAT CCTGCAGCAG 481 TAGGTCCCAT GGTCATCCNG AGTCACATTC TTTATGATCA GANACACGTC TCCTTTGTTG 541 AGATCGCNCT TTANCTGGTA TCTGCTGGAN TTCTNATATG TCACATTTCT TTCNTCAGTT 601 CTGANCNACT CGTTGGTACA CTGNGANNNN NNNNNN //
Primer: hGCSF_R (Pr0031) Region: IgG Fc-region Sequence file: RDB18987_B1Drc.seq
>D07990C6_B1Dr_1_hGCSF_R_B05_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GTGGGGNNCC TCNNTCTAGA GTCGCGGCCG 61 CTCTAGGGCA GTGGTGGGTG CTTTATTTCC ATGCTGGGTG CCTGGGAAGT ATGTAGACGG 121 GGTACGTGCC AAGCATCCTC GTGCGACCGC GAGAGCCCGG GGAGCGGGGG CTTGCCGGCC 181 GTCGCACTCA TTTACCCGGG GACAGGGAGA GGCTCTTCTG CGTGTAGTGG TTGTGCAGAG 241 CCTCATGCAT CACGGAGCAT GAGAAGACGT TCCCCTGCTG CCACCTGCTC TTGTCCACGG 301 TGAGCTTGCT ATAGAGGAAG AAGGAGCCGT CGGAGTCCAG CACGGGAGGC GTGGTCTTGT 361 AGTTGTTCTC CGGCTGCCCA TTGCTCTCCC ACTCCACGGC GATGTCGCTG GGATAGAAGC 421 CTTTGACCAG GCAGGTCAGG CTGACCTGGT TCTTGGTCAT CTCCTCCCGG GATGGGGGCA 481 GGGTGTACAC CTGTGGTTCT CGGGGCTGCC CTGTAGGGAC AGAGGTTGGT ACAGCGGTCA 541 CTCTCAGGGC AGAGGGTGGG CCGAGCCGGC CTCTGTCCAT GTGGCCCTCG CACCCCACGG 601 GTCCCACCTT TGGCTTTGGA GATGGTTTTC TCGATGGGGG CTGGGAGGGC TTTGTTGGAG 661 ACCTTGCACT TGTACTCCTT GCCATTCAGC CAGTCCTGGT GCAGGACGGT GAGGACGCTG 721 ACCACACGGT ACGTGCTGTT GTACTGCTCC TCCCGCGGCT TTGTCTTGNC ATTATGCACC 781 TCCACGCCGT CCACGTACCA GTTGAACTTG ACCTCAGGGT CTTCGTGGCT CACGTCCACC 841 ACCNCGCATG TGACCTCNNG GGNNCGGGAN ATCATGANGG TGNNCTTGGG TTTTGGGGGG 901 AAGAGGAAGN NTGACGGTCC CCCCNNNANT TCANNNGCTN ANNANNANAN GGANGTGGAN 961 GTGNCANCNC CCGGNNGGGG NCTGNNCCNN NNNGCNNNNT ANTCNNNNGG NANCNNNNCN 1021 NNCNTNGANC NNNNNNNNNN //
Primer: Fc-CH2M_R (Pr1251) Region: IgG Fc-region Sequence file: RDB18987_B1Drd.seq
>D07990C6_B1Dr_1_Fc-CH2M_R_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NNGCTGNNNN CNCGGNNNCG TGCTGTTGTA 61 CTGCTCCTCC CGCGGCTTTG TCTTGGCATT ATGCACCTCC ACGCCGTCCA CGTACCAGTT 121 GAACTTGACC TCAGGGTCTT CGTGGCTCAC GTCCACCACC ACGCATGTGA CCTCAGGGGT 181 CCGGGAGATC ATGAGGGTGT CCTTGGGTTT TGGGGGGAAG AGGAAGACTG ACGGTCCCCC 241 CAGGAGTTCA GGTGCTGAGG AAGAGATGGA GGTGGACGTG TCAGCACCCG GCTGGGGCCT 301 GTCCCTGGAT GCAGGCTACT CTAGGGCACC TGTCCCGCCT TGAGCTGGAG GGCGAGGCCT 361 GGGCTGGCTT ACCTGGGCAC GGTGGGCATG TGTGAGTTTT GTCACAAGAT TTGGGCTCTG 421 CAGAGAGAAG ATTGGGAGTT ACTGGAATCT GGGAGGAGAG AAGGTGTCCG AGCTGAGGGA 481 GTGGAGAGTT TGGCCTTTGG GGTGGGCTTA GGTCAGGGGC AGGGTCCTCC CGGATATGGC 541 TCTTGGCAGG TCTGAGCCCA GCACCTGCCC CTTTGTGTGC AGGGCCTGGG TTAGGGGCAC 601 CTAGCCTGTG CCTGCCCAGA GCCTGGGGAA AAAGCCAGAA GACCCTCTCC CTGAGNNNNN 661 NTGGGGCGGG CAGAGGCCTC CGGGTGAAGA GGCAGACGGG GCCTGNNNNG CTGCCCTNNN 721 NNGGGACNNN NNNGCCGGGA TGCGTCCAGN NNNGATAGAA NNTANTGGAT CCCCCNGGCT 781 GCAGGAATTC NATATNNTTC TTACTTGTNN NNNNGNTNAT TATGGAGGGG TCANCNNNNG 841 TCTGTGTCTC TGAACCCATT TCTCTNCCGT GGTTAGGGNN NNNNGGAGAA NNCTGTNNNA 901 NANTCCCCAN NNGGCAGTCT NAANCNNNNN NNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,SV40 pro_ori,lacO,lac pro Sequence file: RDB18987_B1Dre.seq
>D07990C6_B1Dr_1_EF1a-N_R_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNGNNNNNN NNNGGGNGNN GTGCGCTCTG CCNNCTGACG GGCACCGGAN 61 CCAATTCCCA CTCCTTTCAA GACCTAGAAG GTCCATTAGC TGCAAAGATT CCTCTCTGTT 121 TAAAACTTTA TCCATCTTTG CAAAGCTTTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC 181 CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA 241 AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG 301 GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC 361 TGCCTGCTGG GGAGGGAATT AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA 421 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC 481 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC 541 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC 601 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT 661 CGGCTGCGGC GAGCGGTATC ANCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 721 GGGGATAACN CANGAAAAGA ACATGTGAGC AAAANGNCAG CAAAAGGNCA GGANCCGTAA 781 AAAGGCCNCG TTGCTGGCGT TTTTCCATAG GCTCCNCCCC CCTGACGAGC ATCACAAAAA 841 TCNACGCTCA NGTCAGANGT GGNNNANCCN NNCNNGACTA TAAAGANNNN AGGCGTTTNC 901 CCCTGGNANC TCCCTCNTGN GCTCTCCNGT NNNNNNCCCT GCCGCTTNNC NGATNNCNNG 961 NNCNNNNNTC TCCCNTNNGG NAGCGTNGNN NNTTTCTCNT AGCTNANNCT GTNNNNNNNN 1021 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Miyanishi, M., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135. [link to RRC of NBRP]

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Other resources in our bank

mouse Havcr2

External Database
mouse Havcr2

Reference sequence