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pEF-Fc (#RDB18974)

Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region.

Clone info. Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region. Please see Suda, T. and Nagata., J. Exp. Med. 179 (3): 873-878, 1994. Transfection of this plasmid into mammalian cells (293 T or COS7 cells) produces a soluble chimeric protein consisting of the extracellular region of membrane protein fused to human IgG Fc region.
Comment This clone contains a genomic DNA fragment corresponding to the 105743170nt to 105741000nt of NC_000014.9. Known differences (at least) : C to T substitution at 105742694nt (in intron). ; T to G substitution at 105741749nt (Asp to Glu). ; C to A substitution at 105741745nt (Leu to Met). ; C to T substitution at 105741596nt (Tyr, synonym). ; A to G substitution at 105741506nt (Thr, synonym). ; T to C substitution at 105741485nt (Ser, synonym). ; C to G substitution at 105741468nt (in intron). ; A to G substitution at 105741443nt (in intron). ; G to C substituiton at 105741434nt (in intron). ; G to A substitution at 105741427nt (in intron). ; G to C substitution at 105741401nt (in intron) ; G to C substitution at 105741359nt (in intron). ; G to A substitution at 105741357nt (in intron). ; T to G substitution at 105741354nt (in intron).
Vector backbone pEF-BOS (RDB07939) (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human IgG1
Depositor|Developer Nagata, Shigekazu |
 
Sequence (full) RDB18974hts01.seq checkAssembled from experimentally sequenced data.
Publication Suda, T., J. Exp. Med. 179 (3): 873-879 (1994). [link to RRC of NBRP]
Test sheet RDB18974_B2Kop1-2.pdf 
 

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Suda, T. and Nagata, S., J. Exp. Med. 179 (3): 873-878, 1994). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Suda, T. and Nagata, S., J. Exp. Med. 179 (3): 873-878, 1994)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18974 pEF-Fc DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pEF-Fc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18974).

Reference section:

Suda, T., Nagata, S., Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179 (3): 873-879 (1994). PMID 7509364. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Suda, T., Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179 (3): 873-879 (1994). PMID 7509364. [link to RRC of NBRP]