RDB18974 - pEF-Fc

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pEF-Fc

Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region.

Catalog number	RDB18974
Resource name	pEF-Fc
Clone info.	Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region. Please see Suda, T. and Nagata., J. Exp. Med. 179 (3): 873-878, 1994. Transfection of this plasmid into mammalian cells (293 T or COS7 cells) produces a soluble chimeric protein consisting of the extracellular region of membrane protein fused to human IgG Fc region.
Comment	This clone contains a genomic DNA fragment corresponding to the 105743170nt to 105741000nt of NC_000014.9. Known differences (at least) : C to T substitution at 105742694nt (in intron). ; T to G substitution at 105741749nt (Asp to Glu). ; C to A substitution at 105741745nt (Leu to Met). ; C to T substitution at 105741596nt (Tyr, synonym). ; A to G substitution at 105741506nt (Thr, synonym). ; T to C substitution at 105741485nt (Ser, synonym). ; C to G substitution at 105741468nt (in intron). ; A to G substitution at 105741443nt (in intron). ; G to C substituiton at 105741434nt (in intron). ; G to A substitution at 105741427nt (in intron). ; G to C substitution at 105741401nt (in intron) ; G to C substitution at 105741359nt (in intron). ; G to A substitution at 105741357nt (in intron). ; T to G substitution at 105741354nt (in intron).
Vector backbone	pEF-BOS (RDB07939) (plasmid)
Selectable markers	Ampicillin
Gene/insert name	human IgG1
Depositor\|Developer	Nagata, Shigekazu \|

Other clones in our bank

External Database
human IgG1

Reference sequence

Sequence (full)	RDB18974zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Suda, T. and Nagata, S., J. Exp. Med. 179 (3): 873-878, 1994). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Suda, T. and Nagata, S., J. Exp. Med. 179 (3): 873-878, 1994). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Suda, T. and Nagata, S., J. Exp. Med. 179 (3): 873-878, 1994)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

提供条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Suda, T. and Nagata, S., J. Exp. Med. 179 (3): 873-878, 1994)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

提供依頼

提供依頼書 [Word

]
提供同意書 (MTA, 非営利学術目的用)[Word

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18974	pEF-Fc	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-Fc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18974).

Reference section:

Suda, T., Nagata, S., Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179 (3): 873-879 (1994). PMID 7509364. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18974_B1Dkp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,MCS,insert 5' Sequence file: RDB18974_B1Dka.seq
>D07990B5_B1Dk_1_EF1a-C_F_A08_02_ABI24.ab1 1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTNNTTCT CNAGCCTCAG ACAGTGGTTC 61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGATCC CTCGACCTCG 121 AGATCCATCG GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCTG 181 CAGCCCGGGG GATCCACTAG TTCTATCCTG CCTGGACGCA TCCCGGCTAT GCAGTCCCAG 241 TCCAGGGCAG CAAGGCAGGC CCCGTCTGCC TCTTCACCCG GAGGCCTCTG CCCGCCCCAC 301 TCATGCTCAG GGAGAGGGTC TTCTGGCTTT TTCCCCAGGC TCTGGGCAGG CACAGGCTAG 361 GTGCCCCTAA CCCAGGCCCT GCACACAAAG GGGCAGGTGC TGGGCTCAGA CCTGCCAAGA 421 GCCATATCCG GGAGGACCCT GCCCCTGACC TAAGCCCACC CCAAAGGCCA AACTCTCCAC 481 TCCCTCAGCT CGGACACCTT CTCTCCTCCC AGATTCCAGT AACTCCCAAT CTTCTCTCTG 541 CAGAGCCCAA ATCTTGTGAC AAAACTCACA CATGCCCACC GTGCCCAGGT AAGCCAGCCC 601 AGGCCTCGCC CTCCAGCTCA AGGCGGGACA GGTGCCCTAG AGTAGCCTGC ATCCAGGGAC 661 AGGCCCCAGC CGGGTGCTGA CACGTCCACC TCCATCTCTT CCTCAGCACC TGAACTCCTG 721 GGGGGANCGT CAGTCTTCCT CTTCCCCCCA AAACCCAAGG ACACCCTCAT GATCTCCCGG 781 ACCCCTGAGG TCACATGCGT GGTGNTGGAN GTGAGCCACG AANANCCTGA NGTCAAGTTC 841 AACTGGTACG TGGANGGCGN GGANGTGCAT AATGNCNAGA NAAAGCCGCN GGGANGANCA 901 NTACAACAGC NCGNACCGTG TGGNCAGCGN NNNNANNNTN NTGCNCNNNN ANNNNNTGNA 961 NNGNCANNGN NTANNNNNNN NANNNNCTNN NACNAANNNN NNNNNN //
Primer: hGCSF_R (Pr0031) Region: hG-CSF 3'UTR+pA,insert 3' Sequence file: RDB18974_B1Dkb.seq
>D07990B5_B1Dk_1_hGCSF_R_D08_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GTNCNNGGTG GGGNNCCTCN CTCTAGAGTC GCGGCCGCTC 61 TAGGGCAGTG GTGGGTGCTT TATTTCCATG CTGGGTGCCT GGGAAGTATG TAGACGGGGT 121 ACGTGCCAAG CATCCTCGTG CGACCGCGAG AGCCCGGGGA GCGGGGGCTT GCCGGCCGTC 181 GCACTCATTT ACCCGGGGAC AGGGAGAGGC TCTTCTGCGT GTAGTGGTTG TGCAGAGCCT 241 CATGCATCAC GGAGCATGAG AAGACGTTCC CCTGCTGCCA CCTGCTCTTG TCCACGGTGA 301 GCTTGCTATA GAGGAAGAAG GAGCCGTCGG AGTCCAGCAC GGGAGGCGTG GTCTTGTAGT 361 TGTTCTCCGG CTGCCCATTG CTCTCCCACT CCACGGCGAT GTCGCTGGGA TAGAAGCCTT 421 TGACCAGGCA GGTCAGGCTG ACCTGGTTCT TGGTCATCTC CTCCCGGGAT GGGGGCAGGG 481 TGTACACCTG TGGTTCTCGG GGCTGCCCTG TAGGGACAGA GGTTGGTACA GCGGTCACTC 541 TCAGGGCAGA GGGTGGGCCG AGCCGGCCTC TGTCCATGTG GCCCTCGCAC CCCACGGGTC 601 CCACCTTTGG CTTTGGAGAT GGTTTTCTCG ATGGGGGCTG GGAGGGCTTT GTTGGAGACC 661 TTGCACTTGT ACTCCTTGCC ATTCAGCCAG TCCTGGTGCA GGACGGTGAG GACGCTGACC 721 ACACGGTACG TGCTGTTGTA CTGCTCCTCC CGCGGCTTTG TCTTGGCATT ATGCACCTCC 781 ACGCCGTCCA CGTACCAGTT GAACTTGACC TCANGGTCTT CGTGGCTCAC GTCCACCACC 841 ACGCATGTGN CCTCANGGGT CCGGGANATC ATGANGGNGT CCTTNGGTTT TGGGGGGGAA 901 GAGGAAGANT GANNGNCCCC CCANGANTTC ANGTGCTGAN GAANANNNGN NNGTGGNNGN 961 NNCANCNCCC NGCTGGGNNN TNNNCNNNNN NGCNNNNNAN NNNNNGNNNN NNNNNN //
Primer: Fc-CH2M_R (Pr1251) Region: MCS,insert 5' Sequence file: RDB18974_B1Dkc.seq
>18974_18974_B2IsF_1_Fc-CH2M_R_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGG NGNNGNNGCT GNCCNCACGG TACGTGCTGT TGTACTGCTC 61 CTCCCGCGGC TTTGTCTTGG CATTATGCAC CTCCACGCCG TCCACGTACC AGTTGAACTT 121 GACCTCAGGG TCTTCGTGGC TCACGTCCAC CACCACGCAT GTGACCTCAG GGGTCCGGGA 181 GATCATGAGG GTGTCCTTGG GTTTTGGGGG GAAGAGGAAG ACTGACGGTC CCCCCAGGAG 241 TTCAGGTGCT GAGGAAGAGA TGGAGGTGGA CGTGTCAGCA CCCGGCTGGG GCCTGTCCCT 301 GGATGCAGGC TACTCTAGGG CACCTGTCCC GCCTTGAGCT GGAGGGCGAG GCCTGGGCTG 361 GCTTACCTGG GCACGGTGGG CATGTGTGAG TTTTGTCACA AGATTTGGGC TCTGCAGAGA 421 GAAGATTGGG AGTTACTGGA ATCTGGGAGG AGAGAAGGTG TCCGAGCTGA GGGAGTGGAG 481 AGTTTGGCCT TTGGGGTGGG CTTAGGTCAG GGGCAGGGTC CTCCCGGATA TGGCTCTTGG 541 CAGGTCTGAG CCCAGCACCT GCCCCTTTGT GTGCAGGGCC TGGGTTAGGG GCACCTAGCC 601 TGTGCCTGCC CAGAGCCTGG GGAAAAAGCC AGAAGACCCT CTCCCTGAGC ATGAGTGGGG 661 CGGGCAGAGG CCTCCGGGTG AAGAGGCAGA CGGGGCCTGC CTTGCTGCCC TGGACTGGGA 721 CTGCATAGCC GGGATGCGTC CAGGCAGGAT AGAACTAGTG GATCCCCCGG GCTGCANGAA 781 TTCGATATCA AGCTTATCGA TACCGTCGAC CTCGAGGGGG GGCCCGATGG ATCTCGAGGT 841 CGAGGGATCT CTAGAGAATT CCTCACGACA CCTGAAATGG AAGAAAAAAA CTTTGAACCA 901 CTGTCTGANN NTTGAGAATG AACCNAGATN CAAACTCAAA AAGGGNNNNT CCNNNNAGAA 961 TTACNTCNNG TGNNNNGCTG GNNTNNCTTC NNNNTNNNCC NNNNNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,SV40 pro_ori Sequence file: RDB18974_B1Dkd.seq
>D07990B5_B1Dk_1_EF1a-N_R_B08_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGNCGA NGTGCGCTCT GCCCNCTGAC GGGCACCGGA 61 NCCAATTCCC ACTCCTTTCA AGACCTAGAA GGTCCATTAG CTGCAAAGAT TCCTCTCTGT 121 TTAAAACTTT ATCCATCTTT GCAAAGCTTT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT 181 CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA 241 AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG 301 GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT 361 CTGCCTGCTG GGGAGGGAAT TAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 421 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 481 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 541 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 601 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 661 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CNACAGAATC 721 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGNCAG CAAAAGGCCA GGAANCGTAA 781 AAAGGNCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGANGAGC ATCACAAAAA 841 TCGACGCTCA AGTCANANGT GGCGAAACCC GACAGGACTA TAAAGATACC ANGCGTTNCC 901 CCCNGNAANC TCCCTCGTGC GCTCTCCNGT TNCGANCCTG CCGCTTANNN GATACCTGTC 961 CGCCTTTCNN CNNNCNGGAA GCGTGGCGNT TTCNCATANC TCACGCTGNN NNNATCTCAN 1021 NNCNGNNGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Suda, T., Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179 (3): 873-879 (1994). PMID 7509364. [link to RRC of NBRP]

2023.10.05

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