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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Tim4-Fc (#RDB18988)

Mammalian expression vector of the extracellular region of mouse Timd4 (Tim4) (aa 1-273).

Clone info. Mammalian expression vector of the extracellular region of mouse T cell immunoglobulin and mucin domain containing 4 (Timd4, Aliases: Tim4) (aa 1-273) with a splice donor (GTAAGCC). See Fig.3f of Miyanishi, M. et al. Nature, 450 (7168): 435-439, 2007.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pEF-Fc (RDB18974) (plasmid)
Selectable markers Ampicillin
Gene/insert name mouse Timd4 cDNA
Depositor|Developer Nagata, Shigekazu |
 
Sequence (full) RDB18988zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18988 pEF-Tim4-Fc DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-Tim4-Fc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18988).

Reference section:

Miyanishi, M., Tada, K., Koike, M., Uchiyama, Y., Kitamura, T., Nagata, S., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18988_B1Drp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,insert 5'(mTim4(aa 1-273) 5')
Sequence file: RDB18988_B1Dra.seq check
>D07990C7_B1Dr_3_EF1a-C_F_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTT TGAGTTTGGA NCTTGGTTNN TTCTCNNGCC TCAGACAGTG
   61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTAGAG ATCCCTCGAC
  121 CTCGAGATCC ATCGGGCCCC CCCTCGAGGT CGACTATCAA AATGTCCAAG GGGCTTCTCC
  181 TCCTCTGGCT GGTGACGGAG CTCTGGTGGC TTTATCTGAC ACCAGCTGCC TCAGAGGATA
  241 CAATAATAGG GTTTTTGGGC CAGCCGGTGA CTTTGCCTTG TCATTACCTC TCGTGGTCCC
  301 AGAGCCGCAA CAGTATGTGC TGGGGCAAAG GTTCATGTCC CAATTCCAAG TGCAATGCAG
  361 AGCTTCTCCG TACAGATGGA ACAAGAATCA TCTCCAGGAA GTCAACAAAA TATACACTTT
  421 TGGGGAAGGT CCAGTTTGGT GAAGTGTCCT TGACCATCTC AAACACCAAT CGAGGTGACA
  481 GTGGGGTGTA CTGCTGCCGT ATAGAGGTGC CTGGCTGGTT CAATGATGTC AAGAAGAATG
  541 TGCGCTTGGA GCTGAGGAGA GCCACAACAA CCAAAAAACC AACAACAACC ACCCGGCCAA
  601 CCACCACCCC TTATGTGACC ACCACCACCC CAGAGCTGCT TCCAACAACA GTCATGACCA
  661 CATCTGTTCT CCCAACCACC ACACCACCCC AGACACTAGC CACCACTGCC TTCAGTACAG
  721 CAGTGACCAC GTGCCCCTCA ACAACACCTG GCTCCTTCTC ACAAGAAACC ACAAAAGGGT
  781 CCGCCTTCAC TACAGAATCA GAAACTCTGC CTGCATCCNA TCACTCTCAA AGAANCATGA
  841 TGACCATATC TACAGACATA GCCGTANTCN NGNCCACAGG CTCTAANCCN GGGANTCTCC
  901 CNTCCACTTN NCAGCTGANG ANNCAGAAAA CAACATTAAC NACAAGTGAN TCTTTGCANA
  961 ANANNANTAA NNCACATCNN ANTAAGCCNA NATNNAATTN CNNGNNGCCN NGGGGNNNNN
1021 NNNANNNNNA NNNNNNNNNN
//
Primer: hIgG-genome_R1 (Pr1105)
Region: insert 3'(splice donor site,mTim4(aa 1-273) 3')
Sequence file: RDB18988_B1Drb.seq check
>D07990C7_B1Dr_3_hlgG-genome_R1_H04_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCTGANCC CNGCACCTGC CCCTTTGTGT
   61 GCAGGGCCTG GGTTAGGGGC ACCTAGCCTG TGCCTGCCCA GAGCCTGGGG AAAAAGCCAG
  121 AAGACCCTCT CCCTGAGCAT GAGTGGGGCG GGCAGAGGCC TCCGGGTGAA GAGGCAGACG
  181 GGGCCTGCCT TGCTGCCCTG GACTGGGACT GCATAGCCGG GATGCGTCCA GGCANGATAG
  241 AACTAGTGGA TCCCCCGGGC TGCAGGAATT CGATATCGGC TTACTCTGAT GTGATTTAGT
  301 TGTCTTCTGC AAAGACTCAC TTGTTGTTAA TGTTGTTTTC TGTGTCGTCA GCTGTGAAGT
  361 GGATGGGAGA ATCCCAGGGT TAGAGCCTGT GGGCCTGAGT ACGGCTATGT CTGTAGATAT
  421 GGTCATCATG CTTCTTTGAG AGTGATTGGA TGCAGGCAGA GTTTCTGATT CTGTAGTGAA
  481 GGCGGACCCT TTTGTGGTTT CTTGTGAGAA GGAGCCAGGT GTTGTTGAGG GGCACGTGGT
  541 CACTGCTGTA CTGAAGGCAG TGGTGGCTAG TGTCTGGGGT GGTGTGGTGG TTGGGAGAAC
  601 AGATGTGGTC ATGACTGTTG TTGGAAGCAG CTCTGGGGTG GTGGTGGTCA CATAAGGGGT
  661 GGTGGTTGGC CGGGTGGTTG TTGTTGGTTT TTTGGTTGTT GTGGCTCTCC TCAGCTCCAA
  721 GCGCACATTC TTCTTGACAT CNNTGNAANC ANCCAGGCAC CTCTATACNG CANCAGTACN
  781 CCCCACTGTN NNCTCGATTG GTGNTTGANA NGGTCAAGGA NACTTCNNCN AACTGNACCT
  841 TCCCCAAAGT GNATATTTTG TTGANTNNCT GGAGATGATT CTTGTNNCAT CTGTNNGGAN
  901 AAGCTCTGCN TTGCACTTGN ANTGGGANNT NNANCTTTNN CCNNGCANAT ACTGTTGNNN
  961 CTCTGGNNNN NNNANAGNNN TGACNNGNAA ANTCNCNGGC TGNNNNAAAA CCCTATNNNT
1021 GNATCNNNNN ANGNNNNTGN NGNNNNANAA GNNNNNNNNN N
//
Primer: hGCSF_R (Pr0031)
Region: hG-CSF 3'UTR+pA,IgG Fc-region
Sequence file: RDB18988_B1Drc.seq check
>D07990C7_B1Dr_3_hGCSF_R_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNTCCN NGGTGGGGNN CCTCACTCTA GAGTCGCGGC
   61 CGCTCTAGGG CAGTGGTGGG TGCTTTATTT CCATGCTGGG TGCCTGGGAA GTATGTAGAC
  121 GGGGTACGTG CCAAGCATCC TCGTGCGACC GCGAGAGCCC GGGGAGCGGG GGCTTGCCGG
  181 CCGTCGCACT CATTTACCCG GGGACAGGGA GAGGCTCTTC TGCGTGTAGT GGTTGTGCAG
  241 AGCCTCATGC ATCACGGAGC ATGAGAAGAC GTTCCCCTGC TGCCACCTGC TCTTGTCCAC
  301 GGTGAGCTTG CTATAGAGGA AGAAGGAGCC GTCGGAGTCC AGCACGGGAG GCGTGGTCTT
  361 GTAGTTGTTC TCCGGCTGCC CATTGCTCTC CCACTCCACG GCGATGTCGC TGGGATAGAA
  421 GCCTTTGACC AGGCAGGTCA GGCTGACCTG GTTCTTGGTC ATCTCCTCCC GGGATGGGGG
  481 CAGGGTGTAC ACCTGTGGTT CTCGGGGCTG CCCTGTAGGG ACAGAGGTTG GTACAGCGGT
  541 CACTCTCAGG GCAGAGGGTG GGCCGAGCCG GCCTCTGTCC ATGTGGCCCT CGCACCCCAC
  601 GGGTCCCACC TTTGGCTTTG GAGATGGTTT TCTCGATGGG GGCTGGGAGG GCTTTGTTGG
  661 AGACCTTGCA CTTGTACTCC TTGCCATTCA GCCAGTCCTG GTGCAGGACG GTGAGGACGC
  721 TGACCACACG GTACGTGCTG TTGTACTGCT CCTCCCGCGG CTTTGTCTTG GCATTATGCA
  781 CCTCCACGCC GTCCACGTAC CAGTTGAACT TGACCTCAGG GTCTTCGTGG CTCACGTCCA
  841 CCACCACGCN TGTGANCTCA NGGGTCCGGG ANATCATGAG GGTGTCCTTG GGTTTTGGGG
  901 GGAAGANGAA GANTGANNGN CCCCCNAGGA NTTCNNNGCT GANGAANANA TGGANGTGGA
  961 CGNGTCANCA CCCGGNTGGG GNNTGNCCCN NNNNGNNNNC TANTCTNGGG CNNNNGNNCN
 1021 NNCNTGNNNN NNNNGNNNAN NNCNNNNNTN NNNNANCNTG GGNNCNGNNG GNNNNNNNNN
1081 N
//
Primer: Fc-CH2M_R (Pr1251)
Region: IgG Fc-region,insert 3'(splice donor site, mTim4(aa 1-273) 3')
Sequence file: RDB18988_B1Drd.seq check
>18988_18988_B1Dr_3_Fc-CH2M_R_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNGCTGN NNNCNCGGTA CGTGCTGTTG TACTGCTCCT
   61 CCCGCGGCTT TGTCTTGGCA TTATGCACCT CCACGCCGTC CACGTACCAG TTGAACTTGA
  121 CCTCAGGGTC TTCGTGGCTC ACGTCCACCA CCACGCATGT GACCTCAGGG GTCCGGGAGA
  181 TCATGAGGGT GTCCTTGGGT TTTGGGGGGA AGAGGAAGAC TGACGGTCCC CCCAGGAGTT
  241 CAGGTGCTGA GGAAGAGATG GAGGTGGACG TGTCAGCACC CGGCTGGGGC CTGTCCCTGG
  301 ATGCAGGCTA CTCTAGGGCA CCTGTCCCGC CTTGAGCTGG AGGGCGAGGC CTGGGCTGGC
  361 TTACCTGGGC ACGGTGGGCA TGTGTGAGTT TTGTCACAAG ATTTGGGCTC TGCAGAGAGA
  421 AGATTGGGAG TTACTGGAAT CTGGGAGGAG AGAAGGTGTC CGAGCTGAGG GAGTGGAGAG
  481 TTTGGCCTTT GGGGTGGGCT TAGGTCAGGG GCAGGGTCCT CCCGGATATG GCTCTTGGCA
  541 GGTCTGAGCC CAGCACCTGC CCCTTTGTGT GCAGGGCCTG GGTTAGGGGC ACCTAGCCTG
  601 TGCCTGCCCA GAGCCTGGGG AAAAAGCCAG AAGACCCTCT CCCTGAGCAT GAGTGGGGCG
  661 GGCAGAGGCC TCCGGGTGAA GAGGCAGACG GGGCCTGCCT TGCTGCCCTG GACTGGGACT
  721 GCATAGCCGG GATGCGTCCA GGCAGGATAG AACTAGTGGA TCCCCCGGGC TGCAGGAATT
  781 CGATATCGGC TTACTCTGAT GTGATTTAGT TGTCTTCTGC NAAGACTCAC TTGTTGTTAA
  841 TGNTGTTTTC TGTGTCGTCA GCTGTGAAGT GNATGGGAGA ATCCCNGGGN TAGAGCCTGT
  901 GGGCCTGANT ACNGNTATGT CTGTAGATAT GGNCATCNTG CNTNCNTTGA NAGTGNNNGG
  961 NTGNNNGNNN ANTTTCTGAT TCTGTANTNN NGGNNGNANN NTTTTGTGNT TTCTTGNNAN
1021 ANNNANCCNN GNNNNNNNNA GGGGNNNNNN NNNN
//
Primer: EF1a-N_R (Pr0024)
Region: EF-1 alpha pro,SV40 pro_ori,lacO,lac pro
Sequence file: RDB18988_B1Dre.seq check
>18988_18988_B1Dr_3_EF1a-N_R_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNGNNGTGCG CTCTGCCCNC TGACGGGCAC CGGAGCCNAT
   61 TCCCACTCCT TTCAAGACCT AGAAGGTCCA TTAGCTGCAA AGATTCCTCT CTGTTTAAAA
  121 CTTTATCCAT CTTTGCAAAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC
  181 TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT
  241 AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA
  301 GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT
  361 GCTGGGGAGG GAATTAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG
  421 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG
  481 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
  541 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT
  601 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
  661 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
  721 TAACGCAGGA AAGAACATGT GAGCAAAAGG NCAGCAAAAG GNCAGGAANC GTAAAAAGGC
  781 CGCGTTGCTG GCGTTTTTCC NTAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
  841 CTCAAGTCAN ANGTGNNGAA ACCCGACNGG ANTATAAAGA TACCAGGCGT TTCCCCCTGN
  901 AANCTNCCTC GNGCGCTCTN CTGTTNCNAN CCTGNCGCTT ANNGGANANC TGTCCGCCTT
  961 TNNCCCNNNN GGNANCGNNN NNCTTTCTCN NANCTCNNGC NNNNNNNTCT NANTTNNGNN
1021 GNNNNNCNNN NGNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miyanishi, M., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135. [link to RRC of NBRP]