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12/E06 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 12/E06
NCBI Gene ID 2541910
Gene Symbol rpb5
Protein Name DNA-directed RNA polymerase I, II and III subunit Rpb5
Synonym SPAC23C4.15
RefSeq Protein NP_593187.1
PomBase(1) SPAC23C4.15
KEGG(2) spo:SPAC23C4.15
Depositor-supplied data(3) 12E06
Note 757A:G / 766A:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW004902 SpENT12E06 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW044902 SpFFH12E06 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW084902 SpYFH12E06 confirmation required
user_report - reference Weston, L., Genome-wide screen for cell growth regulators in fission yeast. J. Cell Sci. 130 (12): 2049-2055 (2017). PMID 28476936.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.01

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl