pek1 >
32/E04 - S. pombe Postgenome Database Clone
Plasmid request
| Clone ID at the S. pombe Postgenome Database | 32/E04 |
|---|---|
| NCBI Gene ID | 2540919 |
| Gene Symbol | pek1 |
| Protein Name | MAP kinase kinase Pek1 |
| Synonym | SPBC543.07|skh1,mkk1 |
| RefSeq Protein | NP_596795.1 |
| PomBase(1) | SPBC543.07 |
| KEGG(2) | spo:SPBC543.07 |
| Depositor-supplied data(3) | 32E04 |
| Note |
(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.
(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.
(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.
Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.
Please note that they are GENOMIC clones.
Entry vector
| Catalog number | Clone name | Clone status |
|---|---|---|
| SPW012900 | SpENT32E04 | confirmation required |
FFH Vector (FLAG2-His6-tag clone)
| Catalog number | Clone name | Clone status |
|---|---|---|
| SPW052900 | SpFFH32E04 | confirmation required |
YFH Vector (YFP-FLAG-His6-tag clone)
| Catalog number | Clone name | Clone status |
|---|---|---|
| SPW092900 | SpYFH32E04 | confirmation required |
| user_report - reference | Weston, L., Genome-wide screen for cell growth regulators in fission yeast. J. Cell Sci. 130 (12): 2049-2055 (2017). PMID 28476936. |
|---|
Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).
Please visit 
Data Sheet for the information of vectors and sequence primers.
2023.11.07
pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl