BRC Mouse Genomic DNA | Gene Engineering Division - DNA BANK

General Information

Genomic DNA is prepared in the Gene Engineering Division from mouse strains maintained in BRC Experimental Animal Division. High molecular weight DNA is prepared by phenol-chloroform extraction. The DNA is suitable for amplification by PCR. The concentration and purity of DNA samples are determined spectrophotometrically following electrophoresis. DNA concentrations generally range from 50 to 100 ng/μl. (The concentrations of individual samples are supplied with a data sheet.) The DNAs are stored in TE buffer (10 mM TRIS pH7.5, 1mM EDTA) at -30^oC.
For DNA from mouse strains not on List of DNA, please contact us.

Attention International Transfer – “Veterinary Permission” or “Permit to Import Quarantine Material” may be required for shipment of the biological materials. Please follow guide lines in your country.

Forms for Distribution

Order Form:
Please complete the form with your shipping information including your account number of an international courier
(FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
See detail in Information of Request for Distribution

Note:
The resident of the European Economic Area (EEA) and China, please read Special distribution Information to Residents of the Foreign Countries

Order Form for Credit Card Payment. (Visa or Master Card only) [Word] [Example of order form]

Order Form for Bank Transfer Payment. [Word] [Example of order form]

Material Transfer Agreement (Category I MTA) [Word]

For the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization.

Regarding Section 2(a):
Please write your research purpose in detail. We need description specifically how and for what purpose you are going to use the DNA Bank resource(s). If the information is considered insufficient, we may ask you to add more or rewrite it.
We can check whether the documents are filled out correctly or not in advance. Please email documents to us.
Regarding signature line:
"Authorized Representative" is a person who is responsible for intellectual property rights. We request that the candidate is in one of the following positions, he/she can sign the MTA as the Authorized Representative. For anything unclear, please contact us by email.
- College/University/Graduate School: President, Dean, Director or Head of Department
- Research Institution: Director
- Corporation: President, CEO, Director
- Any officer appointed as Intellectual Property Administrator by the organization
The MTA is a formal contract to be executed between institutions. Therefore, we ask your Authorized Representative to be an authority listed above.

Material Transfer Agreement (Category II MTA) [Word]

For the use of our bioresource in research for the following cases:

For research to be conducted by for-profit organizations.
For collaborative research between for-profit organization and not-for-profit organization.
For research by not-for- organization outsourced and sponsored by for-profit organization.
For for-profit research by not-for-profit organization including R&D with the aim of patent acquisition.

Please visit Information of Request for Distribution and Fee and Payment for further information.

If you are inquiring about the availability of a specific DNA, please give as much information about the strain stock number as possible.
The availability of some DNAs is limited. All requests for these DNAs will be given due consideration, but we cannot assure availability.
For more information contact:

The DNA Bank, RIKEN BioResource Research Center (BRC),
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
E-mail: dna_sec.brc@riken.jp

For mouse strains:

Experimental Animal Division, RIKEN BioResource Research Center (BRC),
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
E-mail: animal.brc@riken.jp

Related Information

Reconstitution of DNA

In the case you received plastic vials contains precipitated DNA with ethanol.
A precipitant might stick to the lid or wall during transportation.
Before opening the tube, please spin down a minute or two at 5,000 g (ca. 7,000 rpm, table top microfuge).
Please remove ethanol with pipet (DO NOT tooch precipitate!)
Immediately after removal of ethanol resuspend the DNA in an appropriate amount of TE buffer (10 mM Tris-Cl, pH 7.5, 1 mM EDTA), and dissolve the DNA pellet by gently tapping with fingers (DO NOT shake vigorously! A little amount of ethanol won’t affect to many applications).
If the DNA is difficult to solve, leave the vial at 4 degreeC overnight to allow the DNA to dissolve.
Once reconstituted in TE buffer, the DNA should be stored at -30 degreeC.

(2014.06.18 T.M.)

2022.03.27

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List of mouse strain

Catalog number:	RDB06977
Species:	Mus musculus molossinus
Strain:	M.MUS-NJL
Designations:	Genomic DNA from RBRC00207
Terms and conditions for distribution:	The following terms and conditions will be requested by the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Lot #:
Shipped:	Approx. 10 ug/vial (Approx. 100 ng/uL)
Confirmation:	Agarose gel electrophoresis