- Resarch Tools of Mitophagy
- List of gene resources
- Resarch Tools of Autophagy (Gene Set Collection) [link]
Resarch Tools of Mitophagy
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag.
Yim, W.W., Yamamoto, H., Mizushima, N.
Elife 11: e78923 (2022). PubMed PMID 35938926.
| Catalog no. | Name of resource | Description |
| RDB19962 | pMRX-IB-pSu9-HaloTag7-mGFP | Retroviral vector for stable expression of pSu9-HaloTag7-mGFP to measure mitophagy activity. |
| RDB19963 | pMRX-IB-HaloTag7-mGFP-KDEL | Retroviral vector for stable expression of HaloTag7-mGFP-KDEL to measure ER-phagy activity. |
Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration.
Katayama, H., Hama, H., Nagasawa, K., Kurokawa, H., Sugiyama, M., Ando, R., Funata, M., Yoshida, N., Homma, M., Nishimura, T., Takahashi, M., Ishida, Y., Hioki, H., Tsujihata, Y., Miyawaki, A. Cell 181 (5): 1176-1187.e16 (2020). PubMed PMID 32437660.
| Catalog no. | Name of resource | Description |
| RDB18222 | SRAI_pcDNA3 | Expression vector of fluorescent protein, SRAI (signal-retaining autophagy indicator) for autophagy sensor. |
| RDB18223 | mito-SRAI_pcDNA3 | Expression vector of fluorescent protein, mito-SRAI (signal-retaining autophagy indicator) for quantitative visualizing mitophagy. |
| RDB18684 | pAAV2-TRE-mito-SRAI-BGHpA | AAV2 expression vector of mito-SRAI (signal-retaining autophagy indicator) driven by the tetracyclin-responsive element promoter. |
Glycolytic suppression dramatically changes the intracellular metabolic profile of multiple cancer cell lines in a mitochondrial metabolism-dependent manner.
Shiratori, R., Furuichi, K., Yamaguchi, M., Miyazaki, N., Aoki, H., Chibana, H., Ito, K., Aoki, S. Sci. Rep. 9 (1): 18699 (2019). PubMed PMID 31822748.
| Catalog no. | Name of resource | Description |
| RDB18369 | mitoKeima-Red | Expression vector encoding mitochondrially targeted Keima-Red (mitoKeima-Red). |
A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.
Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A.
Chem. Biol. 18 (8): 1042-1052 (2011). PubMed PMID 21867919.
| Catalog no. | Name of resource | Description |
| RDB15236 | mKeima (h)/pCS2 | Expression vector of monomeric Keima-Red (mKeima). |
| RDB15237 | dKeima (h)/pCS2 | Expression vector of dimeric Keima-Red (dKeima). |
| RDB15238 | mt-mKeima (h)/pIND(SP1) | Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. |
| RDB15456 | mt-mKeima/pcDNA3 | Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. |
List of gene resources
Link to KEGG Pathway – Mitophagy – Animal Pathway
List of gene resources
- Human [open/close]
AMBRA1 ATF4 ATG5 ATG9A ATG9B BCL2L1 BCL2L13 BECN1 BECN2 BNIP3 BNIP3L CALCOCO2 CITED2 CSNK2A1 CSNK2A2 CSNK2A3 CSNK2B E2F1 EIF2AK3 FIS1 FOXO3 FUNDC1 GABARAP GABARAPL1 GABARAPL2 HIF1A HRAS JUN KRAS MAP1LC3A MAP1LC3B MAP1LC3B2 MAP1LC3C MAPK10 MAPK8 MAPK9 MFN1 MFN2 MITF MRAS NBR1 NRAS OPTN PGAM5 PINK1 PRKN RAB7A RAB7B RELA RHOT1 RHOT2 RPS27A RRAS RRAS2 SP1 SQSTM1 SRC TAX1BP1 TBC1D15 TBC1D17 TBK1 TFE3 TFEB TOMM7 TP53 UBA52 UBB UBC ULK1 USP15 USP30 USP8 - Mouse [open/close]
- KEGG orthology [open/close]
Link to KEGG Pathway – Mitophagy – Yeast Pathway
(GSB0051e, T.M. 2014.10.13)
2023.08.20
