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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

dKeima (h)/pCS2

Expression vector of dimeric Keima-Red (dKeima).

Catalog number RDB15237
Resource name dKeima (h)/pCS2
Clone info. Expression vector of dimeric Keima-Red (dKeima).
Vector backbone pCS2+ (Plasmid)
Gene/insert name Montipora. sp RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Montipora. sp RFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A. (2011) A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. Chemistry & Biology, 18 (8): 1042-1052.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            付加的提供条件:
            本件リソースの使用は学術機関での学術研究に限る。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15237 dKeima (h)/pCS2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The dKeima (h)/pCS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15237).

            Reference section:

            Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19809_B2L5p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pCMV-IE94_F (pr 506)
            Region: CMV IE94 pro,SP6 pro,insert 5'
            Sequence file: RDB19809_B2L5a.seq check
            >D04498K5_B2L5_1_pCMV-IE94_F_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNGNNNNN ATGGGNNGNC TATATAAGCA ATGCTCGTTT AGGGAACCGC
               61 CATTCTGCCT GGGGACGTCG GAGCAAGCTT GATTTAGGTG ACACTATAGA ATACAAGCTA
              121 CTTGTTCTTT TTGCAGGATC CGCCATGGTG AGCGTGATCG CCAAGCAGAT GACCTACAAG
              181 GTGTACATGT CCGGCACCGT GAACGGCCAC TACTTCGAGG TGGAGGGCGA CGGCAAGGGC
              241 AAGCCCTACG AGGGCGAGCA GACCGTGAAG CTGACCGTGA CCAAGGGCGG CCCCCTGCCC
              301 TTCGCCTGGG ACATCCTGTC CCCCCTGTTC CAGTACGGCA GCATCCCCTT CACCAAGTAC
              361 CCCGAGGACA TCCCCGACTA CGTGAAGCAG AGCTTCCCCG AGGGCTACAC CTGGGAGAGG
              421 ACCATGAACT TCGAGGACGG CGCCGTGTGC ACCGTGAGCA ACGACTCCAG CATCCAGGGC
              481 AACTGCTTCA TCTACAACGT GAAGATCAGC GGCACCAACT TCCCCCCCAA CGGCCCCGTG
              541 ATGCAGAAGA AGACCCAGGG CTGGGAGCCC AGCACCGAGA GGCTGTTCGC CAGGGACGGA
              601 ATGCTGATCG GCAACGACTA CATGGCCCTG AAGCTGGAGG GCGGCGGCCA CTACCTGTGC
              661 GAGTTCAAGT CCACCTACAA GGCCAAGAAG CCCGTGAGGN TGCCCGGCTA CCACTACATC
              721 GACAGGAAGC TGGACGTGAC CAGCCACAAC AGGGACTACA CCTCCGTGGA GCAGTGCGAG
              781 ATCGCCATCG CCCGCCACTC CCTGCTGGGC TAAGAATTCA AGGCCTCTCG AGCCTCTAGA
              841 ACTATAGTGA GTCGNATTAC GTAGATCCNG ACATGATAAG ATACATTGAT GANTTNGGNN
              901 AAACCNCAAC TAGAATGCAG NGAAAAAAAN TGCTTTATTN GNGAAANTNG NGNTGCNATN
            961 GCTTTNNTTG NNNNNNNTNA NNNNCNNNNN NNAANNNNNT NNNNNNNNNN
            //
            Primer: reverse-2 (pr 100)
            Region: SV40 pA,insert 3'
            Sequence file: RDB19809_B2L5b.seq check
            >D04498K5_B2L5_1_reverse-2_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNGCNNN GCGCNNNNNN AACCCTCNCT AAAGGGAACA AAAGCTGGGT
               61 ACCGGGCCCA ATGCATTGGC GCCGCGGCCG CGAATTAAAA AACCTCCCAC ACCTCCCCCT
              121 GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA
              181 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA
              241 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCTACGTAAT
              301 ACGACTCACT ATAGTTCTAG AGGCTCGAGA GGCCTTGAAT TCTTAGCCCA GCAGGGAGTG
              361 GCGGGCGATG GCGATCTCGC ACTGCTCCAC GGAGGTGTAG TCCCTGTTGT GGCTGGTCAC
              421 GTCCAGCTTC CTGTCGATGT AGTGGTAGCC GGGCATCCTC ACGGGCTTCT TGGCCTTGTA
              481 GGTGGACTTG AACTCGCACA GGTAGTGGCC GCCGCCCTCC AGCTTCAGGG CCATGTAGTC
              541 GTTGCCGATC AGCATTCCGT CCCTGGCGAA CAGCCTCTCG GTGCTGGGCT CCCAGCCCTG
              601 GGTCTTCTTC TGCATCACGG GGCCGTTGGG GGGGAAGTTG GTGCCGCTGA TCTTCACGTT
              661 GTAGATGAAG CAGTTGCCCT GGATGCTGGA GTCGTTGCTC ACGGTGCACA CGGCGCCGTC
              721 CTCGAAGTTC ATGGTCCTCT CCCAGGTGTA GCCCTCGGGG AAGCTCTGCT TCACGTAGTC
              781 GGGGATGTCC TCGGGGTACT TGGTGAAGGG GATGCTGCCG TACTGGAACA NGGGGGACAG
              841 GATGTCNCAG GCGAANGGCA NGGGGNCGCC CTNNGTCACN GNCAGCTTCA CGGNCTGCTC
              901 GCCNNCNNAG GGCTTGNCNT TGCNNNCGCN NTNNNNNNCN AANTANTGGN NNTTCNNNGN
            961 GNNNGNNNTG NNNNNNNNNN
            //
            Primer: Amp_R (pr 8)
            Region: AmpR pro,f1 ori
            Sequence file: RDB19809_B2L5c.seq check
            >D04498K5_B2L5_1_Amp_R_G02_20_ABI24.ab1
                1 NNNNNNNNNN NNNTNNTTGN NGCATTTATN NGGGTTATTG TCTCATGAGC GGATACATAT
               61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
              121 CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG
              181 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC
              241 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA
              301 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC
              361 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG
              421 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA
              481 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC
              541 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCANGCT
              601 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC NGTCNANCGC
              661 CNATTCNATA TGGCGTATAT GGACTCNTGC CNATNCAATA TGGTGGNTCT GGANCTGTGN
              721 CNATTCNNTA TGNNGTATAT GGNCTCNTGN NNNNTCNNTA TNNNGNNNNT NNACCNNNNC
            781 NNNNTNNNNN TNNNNNANNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Katayama, H., A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem. Biol. 18 (8): 1042-1052 (2011). PMID 21867919. [link to RRC of NBRP]

            2024.03.26

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